Products > Catalog Antibodies > Polyclonal Antibodies

UGT1A1 Rabbit pAb (A1359)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - UGT1A1 Rabbit pAb (A1359)

Western blot analysis of extracts of various cell lines, using UGT1A1 antibody (A1359) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A1359)

Immunofluorescence analysis of L929 cells using UGT1A1 Rabbit pAb (A1359) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for UGT1A1 Rabbit pAb(A1359)

Overview

Product nameUGT1A1 Rabbit pAb
Catalog No.A1359
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human UGT1A1 (NP_000454.1).
SequenceMAVESQGGRPLVLGLLLCVLGPVVSHAGKILLIPVDGSHWLSMLGAIQQLQQRGHEIVVLAPDASLYIRDGAFYTLKTYPVPFQREDVKESFVSLGHNVFENDSFLQRVIKTYKKIKKDSAMLLSGCSHLLHNKELMASLAESSFDVMLTDPFLPCSPIVAQYLSLPTVFFLHALPCSLEFEATQCPNPFSYVPRPLSSH
Gene ID54658
Swiss protP22309
SynonymsUGT1A1; BILIQTL1; GNT1; HUG-BR1; UDPGT; UDPGT 1-1; UGT1; UGT1A; UDPGT1-1
Calculated MW49kDa/59kDa
Observed MW60KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver, Rat liver
Cellular locationEndoplasmic reticulum, Endoplasmic reticulum membrane, Microsome, Single-pass membrane protein
Customer validation

(Mouse liver)

WB(Mus musculus)

ABclonal:Western blot - UGT1A1 Rabbit pAb (A1359) }

Western blot - UGT1A1 Rabbit pAb (A1359)

Western blot analysis of extracts of various cell lines, using UGT1A1 antibody (A1359) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A1359) }

Immunofluorescence - UGT1A1 Rabbit pAb (A1359)

Immunofluorescence analysis of L929 cells using UGT1A1 Rabbit pAb (A1359) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A1359

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A1359 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A1359? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order