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UBC Rabbit pAb (A3207)

Review (1)Publications (7) Datasheet

ABclonal:Western blot - UBC Polyclonal Antibody (A3207)

Western blot analysis of extracts of various cell lines, using UBC antibody (A3207) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - UBC Polyclonal Antibody (A3207)

Immunohistochemistry of paraffin-embedded rat liver using UBC antibody (A3207) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - UBC Polyclonal Antibody (A3207)

Immunohistochemistry of paraffin-embedded human esophageal cancer using UBC antibody (A3207) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - UBC Polyclonal Antibody (A3207)

Immunohistochemistry of paraffin-embedded mouse brain using UBC antibody (A3207) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - UBC Polyclonal Antibody (A3207)

Immunofluorescence analysis of U2OS cells using UBC antibody (A3207) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for UBC Rabbit pAb(A3207)

Overview

Product nameUBC Rabbit pAb
Catalog No.A3207
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBC (NP_066289.3).
SequenceMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
Gene ID7316
Swiss protP0CG48
SynonymsUBC; HMG20; ubiquitin C
Calculated MW77kDa
Observed MW12-55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, BT-474, HepG2, U-251MG, A-549, Mouse testis, Mouse liver, Rat testis, Rat brain
Cellular locationCytoplasm, Nucleus
Customer validation

(Mouse bv2)

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

ABclonal:Western blot - UBC Polyclonal Antibody (A3207) }

Western blot - UBC Polyclonal Antibody (A3207)

Western blot analysis of extracts of various cell lines, using UBC antibody (A3207) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - UBC Polyclonal Antibody (A3207) }

Immunohistochemistry - UBC Polyclonal Antibody (A3207)

Immunohistochemistry of paraffin-embedded rat liver using UBC antibody (A3207) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - UBC Polyclonal Antibody (A3207) }

Immunohistochemistry - UBC Polyclonal Antibody (A3207)

Immunohistochemistry of paraffin-embedded human esophageal cancer using UBC antibody (A3207) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - UBC Polyclonal Antibody (A3207) }

Immunohistochemistry - UBC Polyclonal Antibody (A3207)

Immunohistochemistry of paraffin-embedded mouse brain using UBC antibody (A3207) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - UBC Polyclonal Antibody (A3207) }

Immunofluorescence - UBC Polyclonal Antibody (A3207)

Immunofluorescence analysis of U2OS cells using UBC antibody (A3207) at dilution of 1:100. Blue: DAPI for nuclear staining.

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