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UBC Polyclonal Antibody (A3207)

Review (1)Publications (3) Datasheet

ABclonal:Western blot - UBC antibody (A3207)
ABclonal:Immunohistochemistry - UBC antibody (A3207)
ABclonal:Immunohistochemistry - UBC antibody (A3207)
ABclonal:Immunofluorescence - UBC antibody (A3207)

Overview

Product nameUBC Polyclonal Antibody
Catalog No.A3207
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBC (NP_066289.3).
SequenceMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
Gene ID7316
Swiss protP0CG48
SynonymsHMG20
Calculated MW77kDa
Observed MWRefer to Figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBT-474, HepG2, Mouse testis, Rat brain
Cellular locationCytoplasm, Nucleus
Customer validation

IF(Mouse bv2)

WB(Human, Mouse)

ABclonal:Western blot - UBC antibody (A3207)}

Western blot - UBC antibody (A3207)

Western blot analysis of extracts of various cell lines, using UBC antibody (A3207) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - UBC antibody (A3207)}

Immunohistochemistry - UBC antibody (A3207)

Immunohistochemistry of paraffin-embedded human breast carcinoma using UBC antibody (A3207).
ABclonal:Immunohistochemistry - UBC antibody (A3207)}

Immunohistochemistry - UBC antibody (A3207)

Immunohistochemistry of paraffin-embedded human liver using UBC antibody (A3207) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - UBC antibody (A3207)}

Immunofluorescence - UBC antibody (A3207)

Immunofluorescence analysis of U2OS cells using UBC antibody (A3207) at dilution of 1:100. Blue: DAPI for nuclear staining.

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