Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Tyrosinase Rabbit pAb (A1254)

Publications (9) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Tyrosinase Rabbit pAb (A1254)

Western blot analysis of extracts of Rat brain, using Tyrosinase antibody (A1254) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Tyrosinase Rabbit pAb (A1254)

Immunohistochemistry analysis of paraffin-embedded rat heart using Tyrosinase antibody (A1254) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Tyrosinase Rabbit pAb (A1254)

Immunofluorescence analysis of paraffin-embedded Rat skin using Tyrosinase Rabbit pAb (A1254) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

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Overview

Product name Tyrosinase Rabbit pAb
Catalog No. A1254
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 20-340 of human Tyrosinase (NP_000363.1).
Sequence PWSGDRSPCGQLSGRGSCQNILLSNAPLGPQFPFTGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFWGPNCTERRLLVRRNIFDLSAPEKDKFFAYLTLAKHTISSDYVIPIGTYGQMKNGSTPMFNDINIYDLFVWMHYYVSMDALLGGSEIWRDIDFAHEAPAFLPWHRLFLLRWEQEIQKLTGDENFTIPYWDWRDAEKCDICTDEYMGGQHPTNPNLLSPASFFSSWQIVCSRLEEYNSHQSLCNGTPEGPLRRNPGNHDKSRTPRLPSSADVEFCLSLTQYESGSMDKAANFSFRNTLEGFASPLTGIADAS
Gene ID 7299
Swiss prot P14679
Synonyms ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3; Tyrosinase
Calculated MW 60kDa
Observed MW 80kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Rat brain
Cellular location Melanosome membrane, Single-pass type I membrane protein
Customer validation

WB (Mactra, Meretrix meretrix, Mus musculus, Homo sapiens, Sus scrofa)

IF (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Tyrosinase Rabbit pAb images

ABclonal:Western blot - Tyrosinase Rabbit pAb (A1254)}

Western blot - Tyrosinase Rabbit pAb (A1254)

Western blot analysis of extracts of Rat brain, using Tyrosinase antibody (A1254) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Tyrosinase Rabbit pAb (A1254)}

Immunohistochemistry - Tyrosinase Rabbit pAb (A1254)

Immunohistochemistry analysis of paraffin-embedded rat heart using Tyrosinase antibody (A1254) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Tyrosinase Rabbit pAb (A1254)}

Immunofluorescence - Tyrosinase Rabbit pAb (A1254)

Immunofluorescence analysis of paraffin-embedded Rat skin using Tyrosinase Rabbit pAb (A1254) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TYR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TYR. (Distance between topics and target gene indicate popularity.) TYR

* Data provided by citexs.com, for reference only.

Publishing research using A1254? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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