Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

α-Tubulin Rabbit mAb (A6830)

Reviews (5) Publications (7) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - α-Tubulin Rabbit mAb (A6830)

Western blot analysis of various lysates using α-Tubulin Rabbit mAb (A6830) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - α-Tubulin Rabbit mAb (A6830)

Western blot analysis of various lysates using α-Tubulin Rabbit mAb (A6830) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - α-Tubulin Rabbit mAb (A6830)

Immunohistochemistry analysis of α-Tubulin in paraffin-embedded human colon using α-Tubulin Rabbit mAb (A6830) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - α-Tubulin Rabbit mAb (A6830)

Immunohistochemistry analysis of α-Tubulin in paraffin-embedded mouse heart using α-Tubulin Rabbit mAb (A6830) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - α-Tubulin Rabbit mAb (A6830)

Immunohistochemistry analysis of α-Tubulin in paraffin-embedded mouse kidney using α-Tubulin Rabbit mAb (A6830) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - α-Tubulin Rabbit mAb (A6830)

Immunofluorescence analysis of NIH/3T3 cells using α-Tubulin Rabbit mAb (A6830) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - α-Tubulin Rabbit mAb (A6830)

Immunofluorescence analysis of PC-12 cells using α-Tubulin Rabbit mAb (A6830) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - α-Tubulin Rabbit mAb (A6830)

Immunofluorescence analysis of U2OS cells using α-Tubulin Rabbit mAb (A6830) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - α-Tubulin Rabbit mAb (A6830)

Immunoprecipitation analysis of 300 μg extracts from HeLa cells using 3 μg α-Tubulin Rabbit mAb (A6830). Western blot was performed from the immunoprecipitate using α-Tubulin Rabbit mAb (A6830) at a dilution of 1:1000.

Review
ABclonal: review for α-Tubulin Rabbit mAb(A6830)
Review
ABclonal: review for α-Tubulin Rabbit mAb(A6830)
Review
ABclonal: review for α-Tubulin Rabbit mAb(A6830)
Review
ABclonal: review for α-Tubulin Rabbit mAb(A6830)
Review
ABclonal: review for α-Tubulin Rabbit mAb(A6830)

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Overview

Product name α-Tubulin Rabbit mAb
Catalog No. A6830
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC2486

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 349-448 of human α-Tubulin (P68366).
Sequence TGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE
Gene ID 7277
Swiss prot P68366
Synonyms ALS22; TUBA1; H2-ALPHA; α-Tubulin
Calculated MW 50kDa
Observed MW 50kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IHC-P HumanMouseRat
IF/ICC HumanMouseRat
WB HumanMouseRat
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples 293T, Hep G2, HeLa, Mouse brain, Mouse heart, Rat brain
Cellular location cytoplasm, cytoskeleton, cytosol, extracellular exosome, extracellular region, microtubule cytoskeleton
Customer validation

WB (Rattus norvegicus, Mus musculus, Paralichthys olivaceus, Homo sapiens)

IF (Ovis aries, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

α-Tubulin Rabbit mAb images

ABclonal:Western blot - α-Tubulin Rabbit mAb (A6830)}

Western blot - α-Tubulin Rabbit mAb (A6830)

Western blot analysis of various lysates using α-Tubulin Rabbit mAb (A6830) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - α-Tubulin Rabbit mAb (A6830)}

Western blot - α-Tubulin Rabbit mAb (A6830)

Western blot analysis of various lysates using α-Tubulin Rabbit mAb (A6830) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - α-Tubulin Rabbit mAb (A6830)}

Immunohistochemistry - α-Tubulin Rabbit mAb (A6830)

Immunohistochemistry analysis of α-Tubulin in paraffin-embedded human colon using α-Tubulin Rabbit mAb (A6830) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - α-Tubulin Rabbit mAb (A6830)}

Immunohistochemistry - α-Tubulin Rabbit mAb (A6830)

Immunohistochemistry analysis of α-Tubulin in paraffin-embedded mouse heart using α-Tubulin Rabbit mAb (A6830) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - α-Tubulin Rabbit mAb (A6830)}

Immunohistochemistry - α-Tubulin Rabbit mAb (A6830)

Immunohistochemistry analysis of α-Tubulin in paraffin-embedded mouse kidney using α-Tubulin Rabbit mAb (A6830) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - α-Tubulin Rabbit mAb (A6830)}

Immunofluorescence - α-Tubulin Rabbit mAb (A6830)

Immunofluorescence analysis of NIH/3T3 cells using α-Tubulin Rabbit mAb (A6830) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - α-Tubulin Rabbit mAb (A6830)}

Immunofluorescence - α-Tubulin Rabbit mAb (A6830)

Immunofluorescence analysis of PC-12 cells using α-Tubulin Rabbit mAb (A6830) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - α-Tubulin Rabbit mAb (A6830)}

Immunofluorescence - α-Tubulin Rabbit mAb (A6830)

Immunofluorescence analysis of U2OS cells using α-Tubulin Rabbit mAb (A6830) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - α-Tubulin Rabbit mAb (A6830)}

Immunoprecipitation - α-Tubulin Rabbit mAb (A6830)

Immunoprecipitation analysis of 300 μg extracts from HeLa cells using 3 μg α-Tubulin Rabbit mAb (A6830). Western blot was performed from the immunoprecipitate using α-Tubulin Rabbit mAb (A6830) at a dilution of 1:1000.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on TUBA4A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TUBA4A. (Distance between topics and target gene indicate popularity.) TUBA4A

* Data provided by citexs.com, for reference only.

Publishing research using A6830? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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