Product Type > Antibodies > Tag and Loading Control Antibodies

β-Tubulin Rabbit pAb (AC008)

Publications (80) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:β-Tubulin Rabbit pAb
ABclonal:Western blot - β-Tubulin Rabbit pAb (AC008)

Western blot analysis of extracts of various cell lines, using β-Tubulin antibody (AC008) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry of paraffin-embedded human liver using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry of paraffin-embedded human lung cancer using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of C6 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameβ-Tubulin Rabbit pAb
Catalog No.AC008
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human β-Tubulin (NP_006077.2).
SequenceSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPF
Gene ID203068
Swiss protP07437
SynonymsCDCBM6; CSCSC1; M40; OK/SW-cl.56; TUBB1; TUBB5; Beta Tubulin; TUBB; β-Tubulin
Calculated MW50kDa
Observed MW50kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesSH-SY5Y, MCF7, Mouse testis, Mouse brain, Rat testis
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Mus musculus, Crassostrea gigas, Homo sapiens, Rattus norvegicus, Oncorhynchus mykiss, Brachyura, Capra hircus, Eriocheir sinensis H.Milne-Edwards)

IHC(Rattus norvegicus, Mus musculus)

IF(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Homo sapiens, Mus musculus)

Research Area

β-Tubulin Rabbit pAb images

ABclonal:Western blot - β-Tubulin Rabbit pAb (AC008)}

Western blot - β-Tubulin Rabbit pAb (AC008)

Western blot analysis of extracts of various cell lines, using β-Tubulin antibody (AC008) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)}

Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry of paraffin-embedded human liver using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)}

Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry of paraffin-embedded human lung cancer using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)}

Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of C6 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)}

Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)}

Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TUBB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TUBB. (Distance between topics and target gene indicate popularity.) TUBB

* Data provided by citexs.com, for reference only.

Publishing research using AC008? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

Primary Antibodies (2)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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