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β-Tubulin Mouse mAb (AC021)

Publications (21) Datasheet

ABclonal:β-Tubulin Mouse mAb
ABclonal:Western blot - β-Tubulin Mouse mAb (AC021)

Western blot analysis of extracts of various cell lines, using β-Tubulin antibody (AC021) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry of paraffin-embedded rat ovary using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry of paraffin-embedded rat stomach using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameβ-Tubulin Mouse mAb
Catalog No.AC021
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.
ImmunogenRecombinant protein of human β-Tubulin
SequenceEmail for sequence
Gene ID203068
Swiss protP07437
SynonymsCDCBM6; CSCSC1; M40; OK/SW-cl.56; TUBB1; TUBB5; Beta Tubulin; TUBB; β-Tubulin
Calculated MW49kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:5000 - 1:10000
IHC 1:50- 1:200
IF 1:50- 1:200
IP 1:50- 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesSH-SY5Y, MCF-7, HepG2, HeLa, Mouse brain, Mouse lung, Rat brain
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(, Mus musculus, Homo sapiens, Rattus norvegicus, Arabidopsis thaliana)

IP(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - β-Tubulin Mouse mAb (AC021) }

Western blot - β-Tubulin Mouse mAb (AC021)

Western blot analysis of extracts of various cell lines, using β-Tubulin antibody (AC021) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021) }

Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry of paraffin-embedded rat ovary using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021) }

Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry of paraffin-embedded rat stomach using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021) }

Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021) }

Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021) }

Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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