Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A4469)

Datasheet

Tested applications:WBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A4469)

Western blot analysis of extracts of various cell lines, using Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A4469) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameTryptophanyl-tRNA synthetase 1 Rabbit mAb
Catalog No.A4469
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1018
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 3-98 of human Tryptophanyl-tRNA synthetase 1 (P23381).
SequenceNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGI
Gene ID7453
Swiss protP23381
SynonymsGAMMA-2; IFI53; IFP53
Calculated MW53kDa
Observed MW53KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thiomersal, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Mouse testis, Mouse brain, Rat brain
Cellular locationCytoplasm
ABclonal:Western blot - Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A4469)}

Western blot - Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A4469)

Western blot analysis of extracts of various cell lines, using Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A4469) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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