Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody (A2360) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody (A2360).
Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody (A2360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody (A2360) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K9 antibody (A2360). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K9 antibody (A2360) and rabbit IgG. P1 and P2 were located on EBAG9 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
|Product name||TriMethyl-Histone H3-K9 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat, Other (Wide Range)|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:2000|
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
ChIP-seq 1:50 - 1:200
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation|
|Positive samples||HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein|
|Cellular location||Chromosome, Nucleus|
(mouse round spermatid cells, 293, Goat testis, Goat sperm, HEK293T)
WB(Homo sapiens, Mus musculus)
IF(Mus musculus, Homo sapiens)
* For research use only. Not for therapeutic or diagnostic purposes.
Please submit reviews to your technical sales specialist directly. Alternatively, email us at firstname.lastname@example.org
Submit your question about A2360 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.
Publishing research using A2360? Please let us know so that we can cite the reference in this datasheet.