Products > Catalog Antibodies > Methylated Antibodies

TriMethyl-Histone H3-K4 pAb (A2357)

Reviews (8)Publications (5) Datasheet

ABclonal:Western blot - TriMethyl-Histone H3-K4 pAb (A2357)
ABclonal:Dot Blot - TriMethyl-Histone H3-K4 pAb (A2357)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K4 pAb (A2357)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K4 pAb (A2357)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K4 pAb (A2357)
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K4 pAb (A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 pAb(A2357)

Overview

Product nameTriMethyl-Histone H3-K4 pAb
Catalog No.A2357
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsHIST3H3; H3.4; H3/g; H3FT; H3t; histone H3.1t
Calculated MW15kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, H3 protein
Cellular locationChromosome, Nucleus
Customer validation

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WB(Rat)

IF(Human)

IHC(Rat)

IF(Rat)

WB(Rat)

IP(Human)

ChIP-seq(Mouse)

ABclonal:Western blot - TriMethyl-Histone H3-K4 pAb (A2357) }

Western blot - TriMethyl-Histone H3-K4 pAb (A2357)

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K4 antibody (A2357).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Dot Blot - TriMethyl-Histone H3-K4 pAb (A2357) }

Dot Blot - TriMethyl-Histone H3-K4 pAb (A2357)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (A2357) at 1:1000 dilution.
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K4 pAb (A2357) }

Immunohistochemistry - TriMethyl-Histone H3-K4 pAb (A2357)

Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K4 antibody (A2357) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K4 pAb (A2357) }

Immunohistochemistry - TriMethyl-Histone H3-K4 pAb (A2357)

Immunohistochemistry of paraffin-embedded human rectal cancer using TriMethyl-Histone H3-K4 antibody (A2357) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K4 pAb (A2357) }

Immunofluorescence - TriMethyl-Histone H3-K4 pAb (A2357)

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K4 antibody (A2357). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K4 pAb (A2357) }

Chromatin Immunoprecipitation - TriMethyl-Histone H3-K4 pAb (A2357)

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody (A2357) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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