Products > Catalog Antibodies > Methylated Antibodies

TriMethyl-Histone H3-K36 Rabbit pAb (A2366)

Reviews (3)Publications (7) Datasheet

ABclonal:TriMethyl-Histone H3-K36 Rabbit pAb
ABclonal:Western blot - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)
ABclonal: - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)
Review
ABclonal: review for TriMethyl-Histone H3-K36 Rabbit pAb(A2366)
Review
ABclonal: review for TriMethyl-Histone H3-K36 Rabbit pAb(A2366)
Review
ABclonal: review for TriMethyl-Histone H3-K36 Rabbit pAb(A2366)

Overview

Product nameTriMethyl-Histone H3-K36 Rabbit pAb
Catalog No.A2366
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
ChIPseq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

(Hippocampus, HEK293T)

WB(Rattus norvegicus, Homo sapiens, Yeast, Mus musculus)

IHC(Rattus norvegicus)

IF(Rattus norvegicus)

IHC(Sus scrofa)

IF(Homo sapiens)

ABclonal:Western blot - TriMethyl-Histone H3-K36 Rabbit pAb (A2366) }

Western blot - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K36 antibody (A2366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal: - TriMethyl-Histone H3-K36 Rabbit pAb (A2366) }

- TriMethyl-Histone H3-K36 Rabbit pAb (A2366)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K36 antibody (A2366).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K36 Rabbit pAb (A2366) }

Immunohistochemistry - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)

Immunohistochemistry of paraffin-embedded Human breast cancer using TriMethyl-Histone H3-K36 Rabbit pAb (A2366) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K36 Rabbit pAb (A2366) }

Immunohistochemistry - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)

Immunohistochemistry of paraffin-embedded Mouse brain using TriMethyl-Histone H3-K36 Rabbit pAb (A2366) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K36 Rabbit pAb (A2366) }

Immunofluorescence - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K36 antibody (A2366). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K36 Rabbit pAb (A2366) }

Chromatin Immunoprecipitation - TriMethyl-Histone H3-K36 Rabbit pAb (A2366)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K36 antibody (A2366) and rabbit IgG. P1 and P2 were located on GAPDH gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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