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TriMethyl-Histone H3-K36 Rabbit mAb (A20379)

Datasheet

ABclonal:Western blot - TriMethyl-Histone H3-K36 Rabbit mAb (A20379)

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K36 antibody (A20379) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal: - TriMethyl-Histone H3-K36 Rabbit mAb (A20379)

Dot-blot analysis of all sorts of peptides using TriMethyl-Histone H3-K36 antibody (A20379) at 1:1000 dilution.

ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K36 Rabbit mAb (A20379)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K36 antibody (A20379) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameTriMethyl-Histone H3-K36 Rabbit mAb
Catalog No.A20379
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic trimethylated peptide around K36 of human Histone H3 (NP_003520.1).
SequenceEmail for sequence
Gene ID8290/ 8350
Swiss protQ16695/P68431
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionDB 1:500 - 1:2000
WB 1:500 - 1:2000
ChIP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, NIH/3T3, C6
Cellular location
    ABclonal:Western blot - TriMethyl-Histone H3-K36 Rabbit mAb (A20379) }

    Western blot - TriMethyl-Histone H3-K36 Rabbit mAb (A20379)

    Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K36 antibody (A20379) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal: - TriMethyl-Histone H3-K36 Rabbit mAb (A20379) }

    - TriMethyl-Histone H3-K36 Rabbit mAb (A20379)

    Dot-blot analysis of all sorts of peptides using TriMethyl-Histone H3-K36 antibody (A20379) at 1:1000 dilution.
    ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K36 Rabbit mAb (A20379) }

    Chromatin Immunoprecipitation - TriMethyl-Histone H3-K36 Rabbit mAb (A20379)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K36 antibody (A20379) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

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