Products > Catalog Antibodies > Methylated Antibodies

TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Reviews (11)Publications (23) Datasheet

ABclonal:Western blot - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal: - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
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ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)
Review
ABclonal: review for TriMethyl-Histone H3-K27 Rabbit pAb(A2363)

Overview

Product nameTriMethyl-Histone H3-K27 Rabbit pAb
Catalog No.A2363
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
ChIPseq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3
Cellular locationChromosome, Nucleus
Customer validation

(Human embryonic stem cells, A549, Mouse embryonic stem cells, Rice, HeLa, HEK293T, Mouse lung tumor, Goat sperm, Mouse E16.5 neocortex)

ChIP(Oryza sativa, Rattus norvegicus, Gossypium)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

ChIP(Homo sapiens, Gossypium)

IHC(Sus scrofa, Mus musculus, Homo sapiens)

IF(Homo sapiens)

ChIP-Seq(Zea mays)

IP(Gossypium)

ChIP-qPCR(Homo sapiens, Mus musculus)

ABclonal:Western blot - TriMethyl-Histone H3-K27 Rabbit pAb (A2363) }

Western blot - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (A2363) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal: - TriMethyl-Histone H3-K27 Rabbit pAb (A2363) }

- TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A2363).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363) }

Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363) }

Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Immunohistochemistry of paraffin-embedded human breast cancer using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363) }

Immunohistochemistry - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363) }

Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363) }

Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363) }

Immunofluorescence - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Immunofluorescence analysis of U-2 OS cells using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 Rabbit pAb (A2363) }

Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 Rabbit pAb (A2363)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (A2363) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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