Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Publications (12) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal: - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1 μg TriMethyl-Histone H3-K27 Mouse mAb antibody (A16199) , along with a Goat Anti-Mouse IgG (H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3-K27 in representative gene loci (MYOD1), as shown in figure.

ABclonal:Western blot - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Dot Blot - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A16199) at 1:1000 dilution.

ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunohistochemistry analysis of TriMethyl-Histone H3-K27 Mouse mAb in paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunohistochemistry analysis of TriMethyl-Histone H3-K27 Mouse mAb in paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunohistochemistry analysis of TriMethyl-Histone H3-K27 Mouse mAb in paraffin-embedded rat brain using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using TriMethyl-Histone H3-K27 Mouse mAb antibody (A16199) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Overview

Product name TriMethyl-Histone H3-K27 Mouse mAb
Catalog No. A16199
Host species Mouse
Purification method Affinity purification
Isotype IgG1, kappa
CloneNo. AMC0015

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
Sequence Email for sequence
Gene ID 8350
Swiss prot P68431
Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; TriMethyl-Histone H3-K27
Calculated MW 16kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • DB 1:500 - 1:2000
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
  • CUT&Tag 10⁵ cells /1 μg
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, NIH/3T3, C6, H3 protein
Cellular location Chromosome, Nucleus
Customer validation

WB (Mus musculus, Sus scrofa, Homo sapiens)

CHIP (Oryza sativa)

IF (Mus musculus, Rattus norvegicus, R. canina, R. rubiginosa)

ChIP (Oryza sativa, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

TriMethyl-Histone H3-K27 Mouse mAb images

ABclonal: - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

- TriMethyl-Histone H3-K27 Mouse mAb (A16199)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1 μg TriMethyl-Histone H3-K27 Mouse mAb antibody (A16199) , along with a Goat Anti-Mouse IgG (H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3-K27 in representative gene loci (MYOD1), as shown in figure.
ABclonal:Western blot - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

Western blot - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Dot Blot - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

Dot Blot - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A16199) at 1:1000 dilution.
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunohistochemistry analysis of TriMethyl-Histone H3-K27 Mouse mAb in paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunohistochemistry analysis of TriMethyl-Histone H3-K27 Mouse mAb in paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunohistochemistry analysis of TriMethyl-Histone H3-K27 Mouse mAb in paraffin-embedded rat brain using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 Mouse mAb (A16199)}

Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using TriMethyl-Histone H3-K27 Mouse mAb antibody (A16199) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Related Targets

Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4

* Data provided by citexs.com, for reference only.

Publishing research using A16199? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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