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TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Publications (3) Datasheet

ABclonal:Western blot - TriMethyl-Histone H3-K27 Mouse mAb (A16199)
ABclonal: - TriMethyl-Histone H3-K27 Mouse mAb (A16199)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Overview

Product nameTriMethyl-Histone H3-K27 Mouse mAb
Catalog No.A16199
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHepG2, U-87MG, A-375, NIH/3T3, C2C12, C6
Cellular locationChromosome, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens, Sus scrofa)

ABclonal:Western blot - TriMethyl-Histone H3-K27 Mouse mAb (A16199) }

Western blot - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (A16199) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal: - TriMethyl-Histone H3-K27 Mouse mAb (A16199) }

- TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A16199) at 1:1000 dilution.
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199) }

Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K27 antibody (A16199) at dilution of 1:25 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199) }

Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (A16199) at dilution of 1:25 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199) }

Immunohistochemistry - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K27 antibody (A16199) at dilution of 1:25 (40x lens).
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199) }

Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199) }

Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199) }

Immunofluorescence - TriMethyl-Histone H3-K27 Mouse mAb (A16199)

Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 antibody (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.

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