Products > Catalog Antibodies > Methylated Antibodies

TriMethyl-Histone H3-K27 pAb (A2363)

Reviews (10)Publications (12) Datasheet

ABclonal:Western blot - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Dot Blot - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363)
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 pAb (A2363)

Overview

Product nameTriMethyl-Histone H3-K27 pAb
Catalog No.A2363
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa acid extract, NIH/3T3 acid extract, H3 protein
Cellular locationChromosome, Nucleus
Customer validation

ChIP(A549, Mouse embryonic stem cells, HeLa, Human, Rice, Oryza Sativa, Cotton, Rat)

IF(Mouse E16.5 neocortex, Goat sperm, HEK293T, Rice)

IHC(Mouse lung tumor, Pig)

WB(Human, Rat)

ChIP(Human)

IP(Cotton)

CHIP(Cotton)

ABclonal:Western blot - TriMethyl-Histone H3-K27 pAb (A2363) }

Western blot - TriMethyl-Histone H3-K27 pAb (A2363)

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (A2363) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Dot Blot - TriMethyl-Histone H3-K27 pAb (A2363) }

Dot Blot - TriMethyl-Histone H3-K27 pAb (A2363)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A2363).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363) }

Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)

Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363) }

Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)

Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363) }

Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)

Immunohistochemistry of paraffin-embedded human breast cancer using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363) }

Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)

Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363) }

Immunohistochemistry - TriMethyl-Histone H3-K27 pAb (A2363)

Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363) }

Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363)

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K27 antibody (A2363). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363) }

Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363)

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (Red)(A2363) and BrdU antibody (Green)(A1482). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363) }

Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363)

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363) }

Immunofluorescence - TriMethyl-Histone H3-K27 pAb (A2363)

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 pAb (A2363) }

Chromatin Immunoprecipitation - TriMethyl-Histone H3-K27 pAb (A2363)

Chromatin immunoprecipitation analysis extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (A2363) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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