Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Tau Rabbit mAb (A23490)

Datasheet SDS

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Tau Rabbit mAb (A23490)

Western blot analysis of various lysates, using Tau Rabbit mAb (A23490) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - Tau Rabbit mAb (A23490)

Confocal imaging of human brain using Tau Rabbit mAb (A23490, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

ABclonal:Immunofluorescence - Tau Rabbit mAb (A23490)

Confocal imaging of mouse brain using Tau Rabbit mAb (A23490, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

ABclonal:Immunoprecipitation - Tau Rabbit mAb (A23490)

Immunoprecipitation analysis of 300 μg extracts from SH-SY5Y cells using 3 μg Tau antibody (A23490). Western blot was performed from the immunoprecipitate using Tau antibody (A23490) at a dilution of 1:1000.

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    Overview

    Product nameTau Rabbit mAb
    Catalog No.A23490
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG
    CloneNo.ARC58125

    Background

    This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

    Immunogen information

    Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAPT (NM_005910.6)
    SequenceMAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEG
    Gene ID4137
    Swiss protP10636
    SynonymsTAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; tau-40; FTDP-17; PPP1R103; Tau-PHF6
    Calculated MW79kDa
    Observed MW50-80kDa

    Applications

    ReactivityHuman, Mouse, Rat
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:1000 - 1:5000
    • IF/ICC 1:50 - 1:200
    • IP 1:50 - 1:200
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
    Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
    Positive samplesSH-SY5Y, U-251MG, Mouse brain, Rat brain
    Cellular locationCell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, axon, Cytoskeleton, Cytosol

    Research Area

    Tau Rabbit mAb images

    ABclonal:Western blot - Tau Rabbit mAb (A23490)}

    Western blot - Tau Rabbit mAb (A23490)

    Western blot analysis of various lysates, using Tau Rabbit mAb (A23490) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - Tau Rabbit mAb (A23490)}

    Immunofluorescence - Tau Rabbit mAb (A23490)

    Confocal imaging of human brain using Tau Rabbit mAb (A23490, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
    ABclonal:Immunofluorescence - Tau Rabbit mAb (A23490)}

    Immunofluorescence - Tau Rabbit mAb (A23490)

    Confocal imaging of mouse brain using Tau Rabbit mAb (A23490, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
    ABclonal:Immunoprecipitation - Tau Rabbit mAb (A23490)}

    Immunoprecipitation - Tau Rabbit mAb (A23490)

    Immunoprecipitation analysis of 300 μg extracts from SH-SY5Y cells using 3 μg Tau antibody (A23490). Western blot was performed from the immunoprecipitate using Tau antibody (A23490) at a dilution of 1:1000.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on MAPT. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to MAPT. (Distance between topics and target gene indicate popularity.) MAPT

    * Data provided by citexs.com, for reference only.

    Publishing research using A23490? Please let us know so that we can cite the reference in this datasheet.

    Antibodies (26)

    Proteins (2)

    Secondary Antibodies (22)

    * For research use only. Not for therapeutic or diagnostic purposes.

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