Reactivity:Human, Mouse, Rat
Western blot analysis of various lysates, using Tau Rabbit mAb (A23490) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Confocal imaging of human brain using Tau Rabbit mAb (A23490, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
Confocal imaging of mouse brain using Tau Rabbit mAb (A23490, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
|Product name||Tau Rabbit mAb|
|Purification method||Affinity purification|
|Immunogen||A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAPT (NM_005910.6)|
|Synonyms||TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; tau-40; FTDP-17; PPP1R103; Tau-PHF6|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
|Application key||Western blotting Immunofluorescence Immunoprecipitation|
|Positive samples||SH-SY5Y, U-251MG, Mouse brain, Rat brain|
|Cellular location||Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, axon, Cytoskeleton, Cytosol|
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Based on existing publications, the following genes are closely related to research on MAPT. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to MAPT. (Distance between topics and target gene indicate popularity.) MAPT
* Data provided by citexs.com, for reference only.
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* For research use only. Not for therapeutic or diagnostic purposes.