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Tau Rabbit pAb (A0002)

Review (1) Datasheet

ABclonal:Western blot - Tau Rabbit pAb (A0002)
ABclonal:Immunofluorescence - Tau Rabbit pAb (A0002)
ABclonal:Immunofluorescence - Tau Rabbit pAb (A0002)
ABclonal:Immunofluorescence - Tau Rabbit pAb (A0002)
Review
ABclonal: review for Tau Rabbit pAb(A0002)

Overview

Product nameTau Rabbit pAb
Catalog No.A0002
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human Tau (NP_058519.3).
SequenceDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
Gene ID4137
Swiss protP10636
SynonymsDDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU; MAPT; Tau
Calculated MW32-45kDa/78- 80kDa
Observed MW53kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brian
Cellular locationCell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, axon, cytoskeleton, cytosol
Customer validation

(mouse brain)

ABclonal:Western blot - Tau Rabbit pAb (A0002) }

Western blot - Tau Rabbit pAb (A0002)

Western blot analysis of extracts of various cell lines, using Tau antibody (A0002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - Tau Rabbit pAb (A0002) }

Immunofluorescence - Tau Rabbit pAb (A0002)

Immunofluorescence analysis of U-251 MG cells using Tau Rabbit pAb (A0002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Tau Rabbit pAb (A0002) }

Immunofluorescence - Tau Rabbit pAb (A0002)

Immunofluorescence analysis of rat brain using Tau Rabbit pAb (A0002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Tau Rabbit pAb (A0002) }

Immunofluorescence - Tau Rabbit pAb (A0002)

Immunofluorescence analysis of mouse brain using Tau Rabbit pAb (A0002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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