Product Type > Antibodies > Tag and Loading Control Antibodies

α-Tubulin Rabbit pAb (AC007)

Tested applications:WB IHC IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using α-Tubulin antibody (AC007) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of various lysates, using α-Tubulin antibody (AC007) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Immunofluorescence analysis of C6 cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U2OS cells using α-Tubulin antibody (AC007) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.

Overview

Product name	α-Tubulin Rabbit pAb
Catalog No.	AC007
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human α-Tubulin (NP_005991.1).
Sequence	MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
Gene ID	7277
Swiss prot	P68366
Synonyms	TUBA4A; ALS22; H2-ALPHA; TUBA1
Calculated MW	48kDa/49kDa
Observed MW	55kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application key	Western blotting
Positive samples	A-431, HeLa, K-562, Mouse kidney, Mouse brain
Cellular location	Cytoplasm, cytoskeleton
Customer validation	WB(Mus musculus, Brachyura, Sus scrofa, Homo sapiens, Rattus norvegicus, Xenopus laevis, Rana amurensis Boulenger) Co-IP(Homo sapiens) IP(Homo sapiens)

Research Area

α-Tubulin Rabbit pAb images

Western blot - α-Tubulin Rabbit pAb (AC007)

Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Immunofluorescence analysis of C6 cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Immunofluorescence analysis of NIH-3T3 cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Immunofluorescence analysis of U-2 OS cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Confocal immunofluorescence analysis of U2OS cells using α-Tubulin antibody (AC007) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.

Product Protocols

Inquire About This Product

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Related Targets

Based on existing publications, the following genes are closely related to research on TUBA4A. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to TUBA4A. (Distance between topics and target gene indicate popularity.) TUBA4A

* Data provided by citexs.com, for reference only.

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Journal of infectious diseases

Application:

IF:

5.226

Species:

Mus musculus

PMID:

24652493

Title:

Etanercept reduces neuroinflammation and lethality in mouse model of Japanese encephalitis

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Developmental And Comparative Immunology

Application:

IF:

3.636

Species:

Brachyura

PMID:

26868307

Title:

The characterization of hematopoietic tissue in adult Chinese mitten crab Eriocheir sinensis

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

British journal of nutrition

Application:

IF:

3.718

Species:

Sus scrofa

PMID:

28643619

Title:

Dietary leucine supplementation alters energy metabolism and induces slow-to-fast transitions in longissimus dorsi muscle of weanling piglets

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

tumor biology

Application:

IF:

3.355

Species:

Homo sapiens

PMID:

28347237

Title:

Specific tumor-derived CCL2 mediated by pyruvate kinase M2 in colorectal cancer cells contributes to macrophage recruitment in tumor microenvironment

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Reproductive Sciences (Thousand Oaks, Calif.)

Application:

IF:

3.06

Species:

Homo sapiens

PMID:

32046465

Title:

Overexpression of Lin28B Promoted the Proliferation of Adenomyotic Smooth Muscle Cells of the Junctional Zone via Regulating Let-7a

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Cancer Biology & Therapy

Application:

IF:

4.742

Species:

Homo sapiens

PMID:

26382920

Title:

Harmine suppresses homologous recombination repair and inhibits proliferation of hepatoma cells .

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

PeerJ

Application:

IF:

2.984

Species:

Mus musculus

PMID:

28663935

Title:

Differential gene expression analysis by RNA-seq reveals the importance of actin cytoskeletal proteins in erythroleukemia cells.

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Cell Death Dis

Application:

Co-IP

IF:

8.469

Species:

Homo sapiens

PMID:

32934212

Title:

EZH2 reduction is an essential mechanoresponse for the maintenance of super-enhancer polarization against compressive stress in human periodontal ligament stem cells

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Cell Death Dis

Application:

IF:

8.469

Species:

Homo sapiens

PMID:

33311454

Title:

NF-κB-Mediated lncRNA AC007271. 3 as A ceRNA Promotes Carcinogenesis of Oral Squamous Cell Carcinoma by Regulating Slug

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Biochem Biophys Res Commun

Application:

IF:

3.575

Species:

Homo sapiens

PMID:

33631676

Title:

FLOT2 upregulation promotes growth and invasion by interacting and stabilizing EphA2 in gliomas

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Biomolecules

Application:

IF:

4.87

Species:

Homo sapiens

PMID:

33801246

Title:

Eicosapentaenoic Acid Inhibits KRAS Mutant Pancreatic Cancer Cell Growth by Suppressing Hepassocin Expression and STAT3 Phosphorylation

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Mol Ther Oncolytics

Application:

IF:

7.2

Species:

Homo sapiens

PMID:

32637584

Title:

Dual Inhibition of MAPK and JAK2/STAT3 Pathways Is Critical for the Treatment of BRAF Mutant Melanoma

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

J Cancer

Application:

IF:

4.207

Species:

Mus musculus

PMID:

34659548

Title:

Bai-He-Gu-Jin-Tang formula suppresses lung cancer via AKT/GSK3β/β-catenin and induces autophagy via the AMPK/mTORC1/ULK1 signaling pathway

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Transl Pediatr

Application:

IF:

2.488

Species:

Homo sapiens

PMID:

34765479

Title:

Compound heterozygous pathogenic variants in the GALC gene cause infant-onset Krabbe disease

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

RNA Biol

Application:

IF:

4.65

Species:

Mus musculus

PMID:

34843419

Title:

Nucleo-cytoplasmic RNA distribution responsible for maintaining neuroinflammatory microenvironment

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Cellular and molecular neurobiology

Application:

IF:

5.04

Species:

Rattus norvegicus

PMID:

33779883

Title:

NIX Mediates Mitophagy in Spinal Cord Injury in Rats by Interacting with LC3

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IF:

Species:

Homo sapiens

PMID:

33744226

Title:

Rabeprazole suppresses cell proliferation in gastric epithelial cells by targeting STAT3-mediated glycolysis

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Food & nutrition research

Application:

IF:

Species:

Mus musculus

PMID:

35382382

Title:

Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

The EMBO journal

Application:

IF:

14.01

Species:

Homo sapiens

PMID:

33978233

Title:

Paternal USP26 mutations raise Klinefelter syndrome risk in the offspring of mice and humans

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Biochemistry and cell biology = Biochimie et biologie cellulaire

Application:

IF:

3.73

Species:

Xenopus laevis

PMID:

35104156

Title:

Mitochondrial DNA methyltransferases and their regulation under freezing and dehydration stresses in the freeze tolerant wood frog, Rana sylvatica

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Nanoscale

Application:

IF:

8.3

Species:

Homo sapiens

PMID:

34259298

Title:

Stealthy nanoparticles protect endothelial barrier from leakiness by resisting the absorption of VE-cadherin

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.89

Species:

Mus musculus

PMID:

35166533

Title:

Sesamol Reverses Myofiber-Type Conversion in Obese States via Activating the SIRT1/AMPK Signal Pathway

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Homo sapiens,Mus musculus

PMID:

35620283

Title:

Roxadustat, a Hypoxia-Inducible Factor 1α Activator, Attenuates Both Long- and Short-Term Alcohol-Induced Alcoholic Liver Disease

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Molecular medicine reports

Application:

IF:

3.42

Species:

Homo sapiens

PMID:

34227673

Title:

Estrogen 17β‑estradiol accelerates the proliferation of uterine junctional zone smooth muscle cells via the let‑7a/Lin28B axis in adenomyosis

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Gene

Application:

IF:

3.91

Species:

Rana amurensis Boulenger

PMID:

36627089

Title:

TXNIP shuttling-a key molecular link in regulating inflammation and mitochondrial dysfunction in freeze tolerant wood frogs

References for α-Tubulin Rabbit pAb (AC007)

Product:

α-Tubulin Rabbit pAb

Journal:

Frontiers in endocrinology

Application:

IF:

6.05

Species:

Mus musculus

PMID:

36387867

Title:

Involvement of POMC neurons in LEAP2 regulation of food intake and body weight - PMC

Publishing research using AC007? Please let us know so that we can cite the reference in this datasheet.

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Product Type > Antibodies > Tag and Loading Control Antibodies

α-Tubulin Rabbit pAb (AC007)

Overview

Background

Immunogen information

Applications

Research Area

α-Tubulin Rabbit pAb images

Product Protocols

Inquire About This Product

Related Targets

Related Research Area

Antibodies (3)

Primary Antibodies (1)

Secondary Antibodies (26)

Antibodies (3)

Primary Antibodies (1)

Secondary Antibodies (26)

Select your location

Product Type > Antibodies > Tag and Loading Control Antibodies

α-Tubulin Rabbit pAb (AC007)

Overview

Background

Immunogen information

Applications

Research Area

α-Tubulin Rabbit pAb images

Product Protocols

Inquire About This Product

Related Targets

Related Research Area

Antibodies (3)

Primary Antibodies (1)

Secondary Antibodies (26)

Antibodies (3)

Primary Antibodies (1)

Secondary Antibodies (26)