Products > Catalog Antibodies > Tag and Loading Control Antibodies

α-Tubulin Rabbit pAb (AC007)

Publications (7) Datasheet

ABclonal:Western blot - α-Tubulin Rabbit pAb (AC007)
ABclonal:Immunofluorescence - α-Tubulin Rabbit pAb (AC007)
ABclonal:Immunofluorescence - α-Tubulin Rabbit pAb (AC007)
ABclonal:Immunofluorescence - α-Tubulin Rabbit pAb (AC007)
ABclonal:Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Overview

Product nameα-Tubulin Rabbit pAb
Catalog No.AC007
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human α-Tubulin (NP_005991.1).
SequenceMRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
Gene ID7277
Swiss protP68366
SynonymsTUBA4A; ALS22; H2-ALPHA; TUBA1
Calculated MW48kDa/49kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:2000 - 1:10000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesA-431, HeLa, K-562, Mouse kidney, Mouse brain
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Mus musculus, Homo sapiens, Sus scrofa, Brachyura)

ABclonal:Western blot - α-Tubulin Rabbit pAb (AC007) }

Western blot - α-Tubulin Rabbit pAb (AC007)

Western blot analysis of extracts of various cell lines, using α-Tubulin antibody (AC007) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - α-Tubulin Rabbit pAb (AC007) }

Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Immunofluorescence analysis of C6 cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - α-Tubulin Rabbit pAb (AC007) }

Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Immunofluorescence analysis of NIH-3T3 cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - α-Tubulin Rabbit pAb (AC007) }

Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Immunofluorescence analysis of U-2 OS cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - α-Tubulin Rabbit pAb (AC007) }

Immunofluorescence - α-Tubulin Rabbit pAb (AC007)

Confocal immunofluorescence analysis of U2OS cells using α-Tubulin antibody (AC007) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about AC007 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using AC007? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order