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TSC1 Rabbit pAb (A0720)

Reviews (2)Publication (1) Datasheet

ABclonal:Western blot - TSC1 Rabbit pAb (A0720)

Western blot analysis of extracts of various cell lines, using TSC1 antibody (A0720) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TSC1 Rabbit pAb (A0720)

Immunohistochemistry of paraffin-embedded rat pancreatic islet using TSC1 antibody (A0720) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - TSC1 Rabbit pAb (A0720)

Immunohistochemistry of paraffin-embedded human gastric cancer using TSC1 antibody (A0720) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - TSC1 Rabbit pAb (A0720)

Immunohistochemistry of paraffin-embedded mouse pancreatic islet using TSC1 antibody (A0720) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - TSC1 Rabbit pAb (A0720)

Immunofluorescence analysis of NIH-3T3 cells using TSC1 Polyclonal Antibody (A0720) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for TSC1 Rabbit pAb(A0720)
Review
ABclonal: review for TSC1 Rabbit pAb(A0720)

Overview

Product nameTSC1 Rabbit pAb
Catalog No.A0720
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 550-650 of human TSC1 (NP_000359.1).
SequenceQAFTPIDLPCGSADESPAGDRECQTSLETSIFTPSPCKIPPPTRVGFGSGQPPPYDHLFEVALPKTAHHFVIRKTEELLKKAKGNTEEDGVPSTSPMEVLD
Gene ID7248
Swiss protQ92574
SynonymsTSC1; LAM; TSC; hamartin
Calculated MW124kDa/129kDa
Observed MW140-170kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, LO2, Mouse brain, Rat brain
Cellular locationCytoplasm, Membrane, Peripheral membrane protein
Customer validation

(Human chronic myeloid leukemia cells, Human bone marrow cancer cell)

WB(Homo sapiens)

ABclonal:Western blot - TSC1 Rabbit pAb (A0720) }

Western blot - TSC1 Rabbit pAb (A0720)

Western blot analysis of extracts of various cell lines, using TSC1 antibody (A0720) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TSC1 Rabbit pAb (A0720) }

Immunohistochemistry - TSC1 Rabbit pAb (A0720)

Immunohistochemistry of paraffin-embedded rat pancreatic islet using TSC1 antibody (A0720) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - TSC1 Rabbit pAb (A0720) }

Immunohistochemistry - TSC1 Rabbit pAb (A0720)

Immunohistochemistry of paraffin-embedded human gastric cancer using TSC1 antibody (A0720) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - TSC1 Rabbit pAb (A0720) }

Immunohistochemistry - TSC1 Rabbit pAb (A0720)

Immunohistochemistry of paraffin-embedded mouse pancreatic islet using TSC1 antibody (A0720) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - TSC1 Rabbit pAb (A0720) }

Immunofluorescence - TSC1 Rabbit pAb (A0720)

Immunofluorescence analysis of NIH-3T3 cells using TSC1 Polyclonal Antibody (A0720) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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