Products > Catalog Antibodies > Monoclonal Antibodies

TRIM28 Mouse mAb (A9917)

Datasheet

ABclonal:Western blot - TRIM28 Monoclonal Antibody (A9917)

Overview

Product nameTRIM28 Mouse mAb
Catalog No.A9917
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
ImmunogenRecombinant protein of human TRIM28
SequenceEmail for sequence
Gene ID10155
Swiss protQ13263
SynonymsKAP1; PPP1R157; RNF96; TF1B; TIF1B; TRIM28
Calculated MW79kDa/88kDa
Observed MW120kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
ICC 1:50 - 1:200
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    immunocytochemistry    Immunoprecipitation    
Positive samples293T, HepG2, SKOV3, HT-29, Jurkat
Cellular locationNucleus
ABclonal:Western blot - TRIM28 Monoclonal Antibody (A9917) }

Western blot - TRIM28 Monoclonal Antibody (A9917)

Western blot analysis of extracts of various cell lines, using TRIM28 antibody (A9917) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Inquire About This Product

Submit your question about A9917 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Discontinued

Contact us to order