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TRIM28 Mouse mAb (A9917)


ABclonal:Western blot - TRIM28 Monoclonal Antibody (A9917)


Product nameTRIM28 Mouse mAb
Catalog No.A9917
Host speciesMouse
Purification methodAffinity purification
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
ImmunogenRecombinant protein of human TRIM28
SequenceEmail for sequence
Gene ID10155
Swiss protQ13263
SynonymsKAP1; PPP1R157; RNF96; TF1B; TIF1B; TRIM28
Calculated MW79kDa/88kDa
Observed MW120kDa
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
ICC 1:50 - 1:200
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    immunocytochemistry    Immunoprecipitation    
Positive samples293T, HepG2, SKOV3, HT-29, Jurkat
Cellular locationNucleus
ABclonal:Western blot - TRIM28 Monoclonal Antibody (A9917) }

Western blot - TRIM28 Monoclonal Antibody (A9917)

Western blot analysis of extracts of various cell lines, using TRIM28 antibody (A9917) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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