Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TRIM21/SS-A Rabbit pAb (A1957)

Publications (2) Datasheet

Tested applications:WBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - TRIM21/SS-A Rabbit pAb (A1957)

Western blot analysis of extracts of various cell lines, using TRIM21/SS-A antibody (A1957) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - TRIM21/SS-A Rabbit pAb (A1957)

Immunohistochemistry of paraffin-embedded mouse spinal cord using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TRIM21/SS-A Rabbit pAb (A1957)

Immunohistochemistry of paraffin-embedded rat kidney using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TRIM21/SS-A Rabbit pAb (A1957)

Immunofluorescence analysis of NIH/3T3 cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TRIM21/SS-A Rabbit pAb (A1957)

Immunofluorescence analysis of PC-12 cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TRIM21/SS-A Rabbit pAb (A1957)

Immunofluorescence analysis of U2OS cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameTRIM21/SS-A Rabbit pAb
Catalog No.A1957
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 86-334 of human TRIM21/SS-A (NP_003132.2).
SequenceGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGA
Gene ID6737
Swiss protP19474
SynonymsTRIM21; RNF81; RO52; Ro/SSA; SSA; SSA1
Calculated MW45kDa/54kDa
Observed MW50KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIF/ICCIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:10 - 1:100
  • IF/ICC 1:10 - 1:100
  • IP 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesJurkat, HeLa, Rat lung
Cellular locationCytoplasm, Cytoplasmic vesicle, Nucleus, P-body, autophagosome
Customer validation

WB(Mus musculus, Homo sapiens)

ABclonal:Western blot - TRIM21/SS-A Rabbit pAb (A1957)}

Western blot - TRIM21/SS-A Rabbit pAb (A1957)

Western blot analysis of extracts of various cell lines, using TRIM21/SS-A antibody (A1957) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - TRIM21/SS-A Rabbit pAb (A1957)}

Immunohistochemistry - TRIM21/SS-A Rabbit pAb (A1957)

Immunohistochemistry of paraffin-embedded mouse spinal cord using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TRIM21/SS-A Rabbit pAb (A1957)}

Immunohistochemistry - TRIM21/SS-A Rabbit pAb (A1957)

Immunohistochemistry of paraffin-embedded rat kidney using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TRIM21/SS-A Rabbit pAb (A1957)}

Immunofluorescence - TRIM21/SS-A Rabbit pAb (A1957)

Immunofluorescence analysis of NIH/3T3 cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TRIM21/SS-A Rabbit pAb (A1957)}

Immunofluorescence - TRIM21/SS-A Rabbit pAb (A1957)

Immunofluorescence analysis of PC-12 cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TRIM21/SS-A Rabbit pAb (A1957)}

Immunofluorescence - TRIM21/SS-A Rabbit pAb (A1957)

Immunofluorescence analysis of U2OS cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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