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TRIF / TICAM1 Rabbit pAb (A1155)

Publications (3) Datasheet

ABclonal:Western blot - TRIF / TICAM1 Polyclonal Antibody (A1155)

Western blot analysis of extracts of various cell lines, using TRIF / TICAM1 antibody (A1155) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameTRIF / TICAM1 Rabbit pAb
Catalog No.A1155
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human TRIF / TICAM1 (NP_891549.1).
SequenceQDKLLYLKHKLKTPRPGCQGQDLLHAMVLLKLGQETEARISLEALKADAVARLVARQWAGVDSTEDPEEPPDVSWAVARLYHLLAEEKLCPASLRDVAYQEAVRTLSSRDDHRLGELQDEARNRCGWDIAGDPGSIRTLQSNLGCLPPSSALPSGTRSLPRPIDGVSDWSQGCSLRSTGSP
Gene ID148022
Swiss protQ8IUC6
SynonymsIIAE6; MyD88-3; PRVTIRB; TICAM-1; TRIF; TRIF/TICAM1; TICAM1
Calculated MW76kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesA-549, U-251MG, 22RV1, Mouse spleen, Mouse eye, Rat spleen
Cellular locationCytoplasmic vesicle, autophagosome
Customer validation

WB(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - TRIF / TICAM1 Polyclonal Antibody (A1155) }

Western blot - TRIF / TICAM1 Polyclonal Antibody (A1155)

Western blot analysis of extracts of various cell lines, using TRIF / TICAM1 antibody (A1155) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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