Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TRAF3 Rabbit pAb (A3094)

Review (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - TRAF3 Rabbit pAb (A3094)

Western blot analysis of extracts of various cell lines, using TRAF3 antibody (A3094) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - TRAF3 Rabbit pAb (A3094)

Immunofluorescence analysis of HeLa cells using TRAF3 antibody (A3094). Blue: DAPI for nuclear staining.

Review
ABclonal: review for TRAF3 Rabbit pAb(A3094)

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Overview

Product name TRAF3 Rabbit pAb
Catalog No. A3094
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. The protein also plays a role in the regulation of antiviral response. Mutations in this are associated with Encephalopathy, acute, infection-induced, herpes-specific 5.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRAF3 (NP_003291.2).
Sequence MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKV
Gene ID 7187
Swiss prot Q13114
Synonyms CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp; RNF118; TRAF3
Calculated MW 64kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:200 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples 22Rv1, SW480, MCF7, U-937, ES-2, Raji, Mouse gastrocnemius muscle, Mouse spleen
Cellular location Cytoplasm, Endosome, Mitochondrion
Customer validation

WB (HeLa)

Documents

Certificate of Compliance

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Lot number

Research Area

TRAF3 Rabbit pAb images

ABclonal:Western blot - TRAF3 Rabbit pAb (A3094)}

Western blot - TRAF3 Rabbit pAb (A3094)

Western blot analysis of extracts of various cell lines, using TRAF3 antibody (A3094) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - TRAF3 Rabbit pAb (A3094)}

Immunofluorescence - TRAF3 Rabbit pAb (A3094)

Immunofluorescence analysis of HeLa cells using TRAF3 antibody (A3094). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TRAF3. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to TRAF3. (Distance between topics and target gene indicate popularity.) TRAF3

* Data provided by citexs.com, for reference only.

Publishing research using A3094? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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