Review (1)Publication (1) Datasheet
Western blot analysis of extracts of various cell lines, using TRADD antibody (A1145) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
| Product name | TRADD Rabbit pAb |
|---|---|
| Catalog No. | A1145 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant protein of mouse TRADD |
|---|---|
| Sequence | Email for sequence |
| Gene ID | 71609 |
| Swiss prot | Q15628 |
| Synonyms | TRADD; Hs.89862 |
| Calculated MW | 34kDa |
| Observed MW | 34kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | MCF7, Jurkat |
| Cellular location | Cytoplasm, Nucleus, cytoskeleton |
| Customer validation | (Human fibrosarcoma cells) WB(Homo sapiens) |
* For research use only. Not for therapeutic or diagnostic purposes.
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