Products > Catalog Antibodies > Polyclonal Antibodies

TRADD Rabbit pAb (A1145)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - TRADD Polyclonal Antibody (A1145)

Western blot analysis of extracts of various cell lines, using TRADD antibody (A1145) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for TRADD Rabbit pAb(A1145)

Overview

Product nameTRADD Rabbit pAb
Catalog No.A1145
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway.
ImmunogenRecombinant protein of mouse TRADD
SequenceEmail for sequence
Gene ID71609
Swiss protQ15628
SynonymsTRADD; Hs.89862
Calculated MW34kDa
Observed MW34kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, Jurkat
Cellular locationCytoplasm, Nucleus, cytoskeleton
Customer validation

(Human fibrosarcoma cells)

WB(Homo sapiens)

    ABclonal:Western blot - TRADD Polyclonal Antibody (A1145) }

    Western blot - TRADD Polyclonal Antibody (A1145)

    Western blot analysis of extracts of various cell lines, using TRADD antibody (A1145) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Submit an review for A1145

    Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

    Reviews List

    Inquire About This Product

    Submit your question about A1145 below and we will get back to you within one business day.

    Alternatively, call us at 888.754.5670.

    Publishing research using A1145? Please let us know so that we can cite the reference in this datasheet.

    Select size and quantity
    5 μL sample size available Add to Cart
    Contact us to order