Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

p53 Rabbit pAb (A0263)

Reviews (2) Publications (65) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - p53 Rabbit pAb (A0263)

Western blot analysis of lysates from MCF7 cells using p53 Rabbit pAb(A0263) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A0263)

Immunohistochemistry analysis of p53 in paraffin-embedded human colon carcinoma using p53 Rabbit pAb (A0263) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - p53 Rabbit pAb (A0263)

Immunoprecipitation of p53 in 200 µg extracts from 293T cells using 3 µg p53 Rabbit pAb (A0263). Western blot analysis was performed using p53 Rabbit pAb (A0263) at 1:3000 dilution.

Review
ABclonal: review for p53 Rabbit pAb(A0263)
Review
ABclonal: review for p53 Rabbit pAb(A0263)

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Overview

Product name p53 Rabbit pAb
Catalog No. A0263
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (NP_000537.3).
Sequence MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
Gene ID 7157
Swiss prot P04637
Synonyms P53; BCC7; LFS1; BMFS5; TRP53; p53
Calculated MW 44kDa
Observed MW 53kDa//53kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples 293T, MCF7, 293T
Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

WB (Human cellsHomo sapiens, Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Megalobrama amblycephala, Sus scrofa, Danio rerio)

IF (SW480Rattus norvegicus, Mus musculus, Homo sapiens, Rattus norvegicus)

IHC (Mus musculus, Rattus norvegicus, Homo sapiens)

Co-IP (Homo sapiens)

ICC (Homo sapiens)

ChIP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

p53 Rabbit pAb images

ABclonal:Western blot - p53 Rabbit pAb (A0263)}

Western blot - p53 Rabbit pAb (A0263)

Western blot analysis of lysates from MCF7 cells using p53 Rabbit pAb(A0263) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A0263)}

Immunohistochemistry - p53 Rabbit pAb (A0263)

Immunohistochemistry analysis of p53 in paraffin-embedded human colon carcinoma using p53 Rabbit pAb (A0263) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - p53 Rabbit pAb (A0263)}

Immunoprecipitation - p53 Rabbit pAb (A0263)

Immunoprecipitation of p53 in 200 µg extracts from 293T cells using 3 µg p53 Rabbit pAb (A0263). Western blot analysis was performed using p53 Rabbit pAb (A0263) at 1:3000 dilution.

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Related Targets

Based on existing publications, the following genes are closely related to research on TP53. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TP53. (Distance between topics and target gene indicate popularity.) TP53

* Data provided by citexs.com, for reference only.

Publishing research using A0263? Please let us know so that we can cite the reference in this datasheet.

Antibodies (31)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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