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TOPBP1 Rabbit pAb (A5781)

Publication (1) Datasheet

ABclonal:Western blot - TOPBP1 Rabbit pAb (A5781)

Western blot analysis of extracts of HCT116 cells, using TOPBP1 antibody (A5781) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TOPBP1 Rabbit pAb (A5781)

Immunohistochemistry of paraffin-embedded mouse heart using TOPBP1 antibody (A5781) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - TOPBP1 Rabbit pAb (A5781)

Immunofluorescence analysis of A549 cells using TOPBP1 antibody (A5781). Blue: DAPI for nuclear staining.

Overview

Product nameTOPBP1 Rabbit pAb
Catalog No.A5781
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2).
SequenceKIITIEETHEELKKQYIFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEEDYEWGSSSILDVLTGINVQQRRLALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSREAGFKRLLQSGGAKVLPGHSVPLFKEATHLFSDLNKLKPDDSGVNIAEAAAQNVYCLRTEYIADYLMQESPPHVENYCLPEAISFIQNNKELGTGLSQKRKAPTEKNKIKRPRVH
Gene ID11073
Swiss protQ92547
SynonymsTOPBP1; TOP2BP1
Calculated MW170kDa
Observed MW200KDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHCT116
Cellular locationChromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Customer validation

WB(Azumapecten farreri)

ABclonal:Western blot - TOPBP1 Rabbit pAb (A5781) }

Western blot - TOPBP1 Rabbit pAb (A5781)

Western blot analysis of extracts of HCT116 cells, using TOPBP1 antibody (A5781) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TOPBP1 Rabbit pAb (A5781) }

Immunohistochemistry - TOPBP1 Rabbit pAb (A5781)

Immunohistochemistry of paraffin-embedded mouse heart using TOPBP1 antibody (A5781) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TOPBP1 Rabbit pAb (A5781) }

Immunofluorescence - TOPBP1 Rabbit pAb (A5781)

Immunofluorescence analysis of A549 cells using TOPBP1 antibody (A5781). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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