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TNNI1 Polyclonal Antibody (A4161)

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ABclonal:Western blot - TNNI1 Polyclonal Antibody (A4161)
Review
ABclonal: review for TNNI1 Polyclonal Antibody(A4161)

Overview

Product nameTNNI1 Polyclonal Antibody
Catalog No.A4161
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human TNNI1 (NP_003272.3).
SequenceMPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLK
Gene ID7135
Swiss protP19237
SynonymsTNNI1; SSTNI; TNN1; troponin I1, slow skeletal type
Calculated MW21kDa
Observed MW22kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse skeletal muscle, Rat skeletal muscle
Cellular location
Customer validation

WB(Pig)

ABclonal:Western blot - TNNI1 Polyclonal Antibody (A4161) }

Western blot - TNNI1 Polyclonal Antibody (A4161)

Western blot analysis of extracts of various cell lines, using TNNI1 antibody (A4161) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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