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TNF-α Rabbit pAb (A11534)

Review (1)Publications (34) Datasheet

ABclonal:TNF-α Rabbit pAb
ABclonal:Western blot - TNF-α Rabbit pAb (A11534)

Western blot analysis of extracts of various cell lines, using TNF-α antibody (A11534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry of paraffin-embedded human lung cancer using TNF-α Rabbit pAb (A11534) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry of paraffin-embedded human thyroid cancer using TNF-α Rabbit pAb (A11534) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A11534)

Immunofluorescence analysis of L929 cells using TNF alpha antibody (A11534) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for TNF-α Rabbit pAb(A11534)

Overview

Product nameTNF-α Rabbit pAb
Catalog No.A11534
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
ImmunogenA synthetic peptide of human TNF
SequenceEmail for sequence
Gene ID7124
Swiss protP01375
SynonymsTNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha
Calculated MW25kDa
Observed MW27KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HT-1080
Cellular locationCell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein
Customer validation

(Mouse breast cancer)

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

IHC(Rattus norvegicus, Mus musculus)

IF(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

ChIP(Homo sapiens,Mus musculus)

ABclonal:Western blot - TNF-α Rabbit pAb (A11534) }

Western blot - TNF-α Rabbit pAb (A11534)

Western blot analysis of extracts of various cell lines, using TNF-α antibody (A11534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534) }

Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry of paraffin-embedded human lung cancer using TNF-α Rabbit pAb (A11534) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534) }

Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry of paraffin-embedded human thyroid cancer using TNF-α Rabbit pAb (A11534) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A11534) }

Immunofluorescence - TNF-α Rabbit pAb (A11534)

Immunofluorescence analysis of L929 cells using TNF alpha antibody (A11534) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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