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TNF-α Rabbit pAb (A0277)

Review (1)Publications (15) Datasheet

ABclonal:Western blot - TNF-α Rabbit pAb (A0277)

Western blot analysis of extracts of various cell lines, using TNF-α antibody (A0277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded Human breast cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded Human gastric cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A0277)

Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody (A0277) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for TNF-α Rabbit pAb(A0277)

Overview

Product nameTNF-α Rabbit pAb
Catalog No.A0277
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human TNF (NP_000585.2).
SequenceMSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Gene ID7124
Swiss protP01375
SynonymsTNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha
Calculated MW25kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1, RAW264.7
Cellular locationCell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein
Customer validation

(Mouse embryonal fibroblast cell)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Sus scrofa, Canis lupus familiaris)

IHC(Mus musculus)

ELISA(Mus musculus)

WB(Mus musculus)

ABclonal:Western blot - TNF-α Rabbit pAb (A0277) }

Western blot - TNF-α Rabbit pAb (A0277)

Western blot analysis of extracts of various cell lines, using TNF-α antibody (A0277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277) }

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277) }

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded Human breast cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277) }

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded Human gastric cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A0277) }

Immunofluorescence - TNF-α Rabbit pAb (A0277)

Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody (A0277) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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