Review (1)Publications (20) Datasheet
Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat
Western blot analysis of extracts of U-937 cells, using TNF-α antibody (A0277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of RAW264.7 cells, using TNF-α antibody (A0277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using TNF-α Rabbit pAb (A0277) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using TNF-α Rabbit pAb (A0277) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat spleen using TNF-α Rabbit pAb (A0277) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody (A0277) at dilution of 1:100. Blue: DAPI for nuclear staining.
| Product name | TNF-α Rabbit pAb |
|---|---|
| Catalog No. | A0277 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TNF (NP_000585.2). |
|---|---|
| Sequence | MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEG |
| Gene ID | 7124 |
| Swiss prot | P01375 |
| Synonyms | TNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha |
| Calculated MW | 25kDa |
| Observed MW | 26KDa/28KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | U-937, RAW264.7, Mouse thymus, Rat thymus |
| Cellular location | Cell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein |
| Customer validation | (Mouse embryonal fibroblast cell) WB(Rattus norvegicus, Homo sapiens, Canis lupus familiaris, Mus musculus, Sus scrofa) ELISA(Mus musculus) WB(Mus musculus) IHC(Mus musculus) IHC(Rattus norvegicus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
