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TNF-α Rabbit pAb (A0277)

Review (1)Publications (20) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - TNF-α Rabbit pAb (A0277)

Western blot analysis of extracts of U-937 cells, using TNF-α antibody (A0277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - TNF-α Rabbit pAb (A0277)

Western blot analysis of extracts of RAW264.7 cells, using TNF-α antibody (A0277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TNF-α Rabbit pAb (A0277) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded human lung cancer using TNF-α Rabbit pAb (A0277) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded rat spleen using TNF-α Rabbit pAb (A0277) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A0277)

Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody (A0277) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for TNF-α Rabbit pAb(A0277)

Overview

Product nameTNF-α Rabbit pAb
Catalog No.A0277
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human TNF (NP_000585.2).
SequenceMSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEG
Gene ID7124
Swiss protP01375
SynonymsTNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha
Calculated MW25kDa
Observed MW26KDa/28KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-937, RAW264.7, Mouse thymus, Rat thymus
Cellular locationCell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein
Customer validation

(Mouse embryonal fibroblast cell)

WB(Rattus norvegicus, Homo sapiens, Canis lupus familiaris, Mus musculus, Sus scrofa)

ELISA(Mus musculus)

WB(Mus musculus)

IHC(Mus musculus)

IHC(Rattus norvegicus)

ABclonal:Western blot - TNF-α Rabbit pAb (A0277)}

Western blot - TNF-α Rabbit pAb (A0277)

Western blot analysis of extracts of U-937 cells, using TNF-α antibody (A0277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - TNF-α Rabbit pAb (A0277)}

Western blot - TNF-α Rabbit pAb (A0277)

Western blot analysis of extracts of RAW264.7 cells, using TNF-α antibody (A0277) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)}

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TNF-α Rabbit pAb (A0277) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)}

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded human lung cancer using TNF-α Rabbit pAb (A0277) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A0277)}

Immunohistochemistry - TNF-α Rabbit pAb (A0277)

Immunohistochemistry of paraffin-embedded rat spleen using TNF-α Rabbit pAb (A0277) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A0277)}

Immunofluorescence - TNF-α Rabbit pAb (A0277)

Immunofluorescence analysis of Human mammary cancer cells using TNF-α antibody (A0277) at dilution of 1:100. Blue: DAPI for nuclear staining.

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