Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

TNF-α Rabbit mAb (A21265)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Immunohistochemistry - TNF-α Rabbit mAb (A21265)

Immunohistochemistry analysis of paraffin-embedded human colon using TNF-α Rabbit mAb (A21265) at dilution of 1:1000(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TNF-α Rabbit mAb (A21265)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using TNF-α Rabbit mAb (A21265) at dilution of 1:1000(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TNF-α Rabbit mAb (A21265)

Immunohistochemistry analysis of paraffin-embedded human lung using TNF-α Rabbit mAb (A21265) at dilution of 1:1000(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

You may also interested in:

Overview

Product name TNF-α Rabbit mAb
Catalog No. A21265
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC52538

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 77-233 of human TNF-α (NP_000585.2).
Sequence VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Gene ID 7124
Swiss prot P01375
Synonyms DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; TNF-α
Calculated MW 26kDa
Observed MW

Applications

Reactivity Human
Tested applications Testing results
IHC-P Human
Recommended dilution
  • IHC-P 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Immunohistochemistry    
Positive samples
Cellular location Membrane, Secreted
Customer validation

IHC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

TNF-α Rabbit mAb images

ABclonal:Immunohistochemistry - TNF-α Rabbit mAb (A21265)}

Immunohistochemistry - TNF-α Rabbit mAb (A21265)

Immunohistochemistry analysis of paraffin-embedded human colon using TNF-α Rabbit mAb (A21265) at dilution of 1:1000(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TNF-α Rabbit mAb (A21265)}

Immunohistochemistry - TNF-α Rabbit mAb (A21265)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using TNF-α Rabbit mAb (A21265) at dilution of 1:1000(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TNF-α Rabbit mAb (A21265)}

Immunohistochemistry - TNF-α Rabbit mAb (A21265)

Immunohistochemistry analysis of paraffin-embedded human lung using TNF-α Rabbit mAb (A21265) at dilution of 1:1000(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Inquire About This Product

Submit your question about A21265 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on TNF. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TNF. (Distance between topics and target gene indicate popularity.) TNF

* Data provided by citexs.com, for reference only.

Publishing research using A21265? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (3)

Proteins (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order