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TNFRSF11B Rabbit pAb (A2100)

Publications (5) Datasheet

ABclonal:Western blot - TNFRSF11B Rabbit pAb (A2100)

Western blot analysis of extracts of Mouse liver, using TNFRSF11B antibody (A2100) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TNFRSF11B Rabbit pAb (A2100)

Immunohistochemistry of paraffin-embedded rat spleen using TNFRSF11B Antibody (A2100) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - TNFRSF11B Rabbit pAb (A2100)

Immunohistochemistry of paraffin-embedded human kidney cancer using TNFRSF11B Antibody (A2100) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - TNFRSF11B Rabbit pAb (A2100)

Immunohistochemistry of paraffin-embedded mouse spleen using TNFRSF11B Antibody (A2100) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - TNFRSF11B Rabbit pAb (A2100)

Immunofluorescence analysis of HeLa cells using TNFRSF11B antibody (A2100). Blue: DAPI for nuclear staining.

Overview

Product nameTNFRSF11B Rabbit pAb
Catalog No.A2100
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-280 of human TNFRSF11B (NP_002537.3).
SequenceETFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYTDSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKHRSCPPGFGVVQAGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGLLLTQKGNATHDNICSGNSESTQKCGIDVTLCEEAFFRFAVPTKFTPNWLSVLVDNLPGTKVNAESVERIKRQHSSQEQTFQLLKLWKHQNKDQDIVKKIIQDIDLCENS
Gene ID4982
Swiss protO00300
SynonymsTNFRSF11B; OCIF; OPG; PDB5; TR1
Calculated MW46kDa
Observed MW48KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver
Cellular locationSecreted
Customer validation

WB(Rattus norvegicus, Mus musculus, Gallus gallus, Homo sapiens)

ABclonal:Western blot - TNFRSF11B Rabbit pAb (A2100) }

Western blot - TNFRSF11B Rabbit pAb (A2100)

Western blot analysis of extracts of Mouse liver, using TNFRSF11B antibody (A2100) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TNFRSF11B Rabbit pAb (A2100) }

Immunohistochemistry - TNFRSF11B Rabbit pAb (A2100)

Immunohistochemistry of paraffin-embedded rat spleen using TNFRSF11B Antibody (A2100) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - TNFRSF11B Rabbit pAb (A2100) }

Immunohistochemistry - TNFRSF11B Rabbit pAb (A2100)

Immunohistochemistry of paraffin-embedded human kidney cancer using TNFRSF11B Antibody (A2100) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - TNFRSF11B Rabbit pAb (A2100) }

Immunohistochemistry - TNFRSF11B Rabbit pAb (A2100)

Immunohistochemistry of paraffin-embedded mouse spleen using TNFRSF11B Antibody (A2100) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - TNFRSF11B Rabbit pAb (A2100) }

Immunofluorescence - TNFRSF11B Rabbit pAb (A2100)

Immunofluorescence analysis of HeLa cells using TNFRSF11B antibody (A2100). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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