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TNFR1/TNFRSF1A Rabbit pAb (A1540)

Publications (5) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Western blot analysis of extracts of various cell lines, using TNFR1/TNFRSF1A antibody (A1540) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunohistochemistry of paraffin-embedded rat lung using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunohistochemistry of paraffin-embedded rat spleen using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunohistochemistry of paraffin-embedded mouse spleen using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunofluorescence analysis of BALB-3T3 cells using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameTNFR1/TNFRSF1A Rabbit pAb
Catalog No.A1540
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 345-455 of human TNFR1/TNFRSF1A (NP_001056.1).
SequenceAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR
Gene ID7132
Swiss protP19438
SynonymsTNFRSF1A; CD120a; FPF; TBP1; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR55; TNFR60; p55; p55-R; p60
Calculated MW24kDa/25kDa/38kDa/50kDa
Observed MW55KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, C6
Cellular locationCell membrane, Golgi apparatus membrane, Secreted, Secreted, Single-pass type I membrane protein
Customer validation

WB(Mus musculus, Homo sapiens, Marine Streptomyces sp)

ABclonal:Western blot - TNFR1/TNFRSF1A Rabbit pAb (A1540) }

Western blot - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Western blot analysis of extracts of various cell lines, using TNFR1/TNFRSF1A antibody (A1540) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540) }

Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunohistochemistry of paraffin-embedded rat lung using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540) }

Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunohistochemistry of paraffin-embedded rat spleen using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540) }

Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunohistochemistry of paraffin-embedded mouse spleen using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TNFR1/TNFRSF1A Rabbit pAb (A1540) }

Immunofluorescence - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunofluorescence analysis of BALB-3T3 cells using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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