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TNFR1 Rabbit pAb (A1540)

Publication (1) Datasheet

ABclonal:Western blot - TNFR1 Rabbit pAb (A1540)
ABclonal:Immunohistochemistry - TNFR1 Rabbit pAb (A1540)
ABclonal:Immunohistochemistry - TNFR1 Rabbit pAb (A1540)
ABclonal:Immunohistochemistry - TNFR1 Rabbit pAb (A1540)
ABclonal:Immunofluorescence - TNFR1 Rabbit pAb (A1540)

Overview

Product nameTNFR1 Rabbit pAb
Catalog No.A1540
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-211 of human TNFR1 (NP_001056.1).
SequenceIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTT
Gene ID7132
Swiss protP19438
SynonymsTNFRSF1A; CD120a; FPF; TBP1; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR55; TNFR60; p55; p55-R; p60
Calculated MW24kDa/25kDa/38kDa/50kDa
Observed MW55KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, Rat kidney
Cellular locationCell membrane, Golgi apparatus membrane, Secreted, Secreted, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - TNFR1 Rabbit pAb (A1540) }

Western blot - TNFR1 Rabbit pAb (A1540)

Western blot analysis of extracts of various cell lines, using TNFR1 antibody (A1540) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00035).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TNFR1 Rabbit pAb (A1540) }

Immunohistochemistry - TNFR1 Rabbit pAb (A1540)

Immunohistochemistry of paraffin-embedded rat lung using TNFR1 Rabbit pAb (A1540) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TNFR1 Rabbit pAb (A1540) }

Immunohistochemistry - TNFR1 Rabbit pAb (A1540)

Immunohistochemistry of paraffin-embedded rat spleen using TNFR1 Rabbit pAb (A1540) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TNFR1 Rabbit pAb (A1540) }

Immunohistochemistry - TNFR1 Rabbit pAb (A1540)

Immunohistochemistry of paraffin-embedded mouse spleen using TNFR1 Rabbit pAb (A1540) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TNFR1 Rabbit pAb (A1540) }

Immunofluorescence - TNFR1 Rabbit pAb (A1540)

Immunofluorescence analysis of BALB-3T3 cells using TNFR1 Rabbit pAb (A1540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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