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TNFAIP3 Rabbit mAb (A19128)

Datasheet

ABclonal:Western blot - TNFAIP3 Rabbit mAb (A19128)
ABclonal:Immunohistochemistry - TNFAIP3 Rabbit mAb (A19128)

Overview

Product nameTNFAIP3 Rabbit mAb
Catalog No.A19128
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]
ImmunogenA synthesized peptide derived from human TNFAIP3
SequenceEmail for sequence
Gene ID7128
Swiss protP21580
SynonymsA20; AISBL; OTUD7C; TNFA1P2; TNFAIP3
Calculated MW82kDa
Observed MW82kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat, HeLa
Cellular location
ABclonal:Western blot - TNFAIP3 Rabbit mAb (A19128) }

Western blot - TNFAIP3 Rabbit mAb (A19128)

Western blot analysis of extracts of various cell lines, using TNFAIP3 antibody (A19128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - TNFAIP3 Rabbit mAb (A19128) }

Immunohistochemistry - TNFAIP3 Rabbit mAb (A19128)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TNFAIP3 Rabbit mAb (A19128) at dilution of 1:100 (40x lens).

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