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TNFAIP3 Rabbit mAb (A19128)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human

ABclonal:Western blot - TNFAIP3 Rabbit mAb (A19128)

Western blot analysis of extracts of various cell lines, using TNFAIP3 antibody (A19128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - TNFAIP3 Rabbit mAb (A19128)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TNFAIP3 Rabbit mAb (A19128) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameTNFAIP3 Rabbit mAb
Catalog No.A19128
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 450-550 of human TNFAIP3 (P21580).
SequencePEESTGGPHSAPPTAPSPFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHNARQLHASHAPDHTRHLDPGKCQACLQDVTRTFNGICSTCFKRTTAEASSS
Gene ID7128
Swiss protP21580
SynonymsA20; AISBL; OTUD7C; TNFA1P2; TNFAIP3
Calculated MW82kDa
Observed MW82kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat, HeLa
Cellular locationCytoplasm, Lysosome, Nucleus
ABclonal:Western blot - TNFAIP3 Rabbit mAb (A19128)}

Western blot - TNFAIP3 Rabbit mAb (A19128)

Western blot analysis of extracts of various cell lines, using TNFAIP3 antibody (A19128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - TNFAIP3 Rabbit mAb (A19128)}

Immunohistochemistry - TNFAIP3 Rabbit mAb (A19128)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TNFAIP3 Rabbit mAb (A19128) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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