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TLR4 Rabbit pAb (A5258)

Reviews (5)Publications (23) Datasheet

ABclonal:Western blot - TLR4 Rabbit pAb (A5258)

Western blot analysis of extracts of Rat heart, using TLR4 antibody (A5258) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - TLR4 Rabbit pAb (A5258)

Western blot analysis of extracts of Mouse heart, using TLR4 antibody (A5258) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TLR4 Rabbit pAb (A5258)

Immunohistochemistry of paraffin-embedded rat spleen using TLR4 antibody (A5258) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - TLR4 Rabbit pAb (A5258)

Immunohistochemistry of paraffin-embedded mouse spleen using TLR4 antibody (A5258) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - TLR4 Rabbit pAb (A5258)

Immunofluorescence analysis of HeLa cells using TLR4 antibody (A5258) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for TLR4 Rabbit pAb(A5258)
Review
ABclonal: review for TLR4 Rabbit pAb(A5258)
Review
ABclonal: review for TLR4 Rabbit pAb(A5258)
Review
ABclonal: review for TLR4 Rabbit pAb(A5258)
Review
ABclonal: review for TLR4 Rabbit pAb(A5258)

Overview

Product nameTLR4 Rabbit pAb
Catalog No.A5258
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500-600 of human TLR4 (NP_612564.1).
SequenceFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQL
Gene ID7099
Swiss protO00206
SynonymsTLR4; ARMD10; CD284; TLR-4; TOLL
Calculated MW73kDa/91kDa/95kDa
Observed MW96KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse heart, Rat heart
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

(Raw264.7, Mouse kidney, Rat spinal cord, Mouse cells, BV2)

IHC(Mus musculus)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Rattus norvegicus, Mus musculus)

IF(Mus musculus)

FC(Homo sapiens)

IF(Mus musculus)

ABclonal:Western blot - TLR4 Rabbit pAb (A5258) }

Western blot - TLR4 Rabbit pAb (A5258)

Western blot analysis of extracts of Rat heart, using TLR4 antibody (A5258) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - TLR4 Rabbit pAb (A5258) }

Western blot - TLR4 Rabbit pAb (A5258)

Western blot analysis of extracts of Mouse heart, using TLR4 antibody (A5258) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TLR4 Rabbit pAb (A5258) }

Immunohistochemistry - TLR4 Rabbit pAb (A5258)

Immunohistochemistry of paraffin-embedded rat spleen using TLR4 antibody (A5258) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TLR4 Rabbit pAb (A5258) }

Immunohistochemistry - TLR4 Rabbit pAb (A5258)

Immunohistochemistry of paraffin-embedded mouse spleen using TLR4 antibody (A5258) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TLR4 Rabbit pAb (A5258) }

Immunofluorescence - TLR4 Rabbit pAb (A5258)

Immunofluorescence analysis of HeLa cells using TLR4 antibody (A5258) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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