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TH Rabbit pAb (A0028)

Publication (1) Datasheet

ABclonal:Western blot - TH Polyclonal Antibody (A0028)
ABclonal:Immunohistochemistry - TH Polyclonal Antibody (A0028)
ABclonal:Immunohistochemistry - TH Polyclonal Antibody (A0028)

Overview

Product nameTH Rabbit pAb
Catalog No.A0028
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human TH (NP_954986.2).
SequenceMPTPDATTPQAKGFRRAVSELDAKQAEAIMVRGQGAPGPSLTGSPWPGTAAPAASYTPTPRSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLE
Gene ID7054
Swiss protP07101
SynonymsDYT14; DYT5b; TYH; TH
Calculated MW44kDa/45kDa/55kDa/56kDa/58kDa
Observed MW59kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular location
Customer validation

WB(Mus musculus)

IHC(Mus musculus)

ABclonal:Western blot - TH Polyclonal Antibody (A0028) }

Western blot - TH Polyclonal Antibody (A0028)

Western blot analysis of extracts of various cell lines, using TH antibody (A0028) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunohistochemistry - TH Polyclonal Antibody (A0028) }

Immunohistochemistry - TH Polyclonal Antibody (A0028)

Immunohistochemistry of paraffin-embedded rat brain using TH Antibody (A0028) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TH Polyclonal Antibody (A0028) }

Immunohistochemistry - TH Polyclonal Antibody (A0028)

Immunohistochemistry of paraffin-embedded mouse spinal cord using TH Antibody (A0028) at dilution of 1:100 (40x lens).

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