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THBS1 Rabbit pAb (A2125)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - THBS1 Rabbit pAb (A2125)

Western blot analysis of extracts of Mouse liver, using THBS1 antibody (A2125) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - THBS1 Rabbit pAb (A2125)

Western blot analysis of extracts of Rat lung, using THBS1 antibody (A2125) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review
ABclonal: review for THBS1 Rabbit pAb(A2125)

Overview

Product nameTHBS1 Rabbit pAb
Catalog No.A2125
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-360 of human THBS1 (NP_003237.2).
SequenceDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSN
Gene ID7057
Swiss protP07996
SynonymsTHBS1; THBS; THBS-1; TSP; TSP-1; TSP1
Calculated MW120kDa/129kDa
Observed MW160KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse liver, Rat lung
Cellular locationEndoplasmic reticulum, Sarcoplasmic reticulum
Customer validation

(unknow)

WB(Homo sapiens)

ABclonal:Western blot - THBS1 Rabbit pAb (A2125) }

Western blot - THBS1 Rabbit pAb (A2125)

Western blot analysis of extracts of Mouse liver, using THBS1 antibody (A2125) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - THBS1 Rabbit pAb (A2125) }

Western blot - THBS1 Rabbit pAb (A2125)

Western blot analysis of extracts of Rat lung, using THBS1 antibody (A2125) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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