Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TGF beta 1 Rabbit pAb (A2124)

Review (1)Publications (55) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - TGF beta 1 Rabbit pAb (A2124)

Western blot analysis of various lysates, using TGF beta 1 antibody (A2124) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review
ABclonal: review for TGF beta 1 Rabbit pAb(A2124)

Overview

Product nameTGF beta 1 Rabbit pAb
Catalog No.A2124
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 291-390 of human TGF beta 1 (NP_000651.3).
SequenceKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
Gene ID7040
Swiss protP01137
SynonymsTGFB1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-β
Calculated MW44kDa
Observed MW12KDa/25KDa/45-60KDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesK-562, Mouse spleen, Rat lung
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

(Rat heart)

IHC(Mus musculus, Rattus norvegicus, Homo sapiens)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa)

IHC(Mus musculus, Rattus norvegicus, Homo sapiens)

IHC(Rattus norvegicus)

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Rattus norvegicus)

ICH(Rattus norvegicus)

ELISA(Homo sapiens)

Research Area

TGF beta 1 Rabbit pAb images

ABclonal:Western blot - TGF beta 1 Rabbit pAb (A2124)}

Western blot - TGF beta 1 Rabbit pAb (A2124)

Western blot analysis of various lysates, using TGF beta 1 antibody (A2124) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Submit an review for A2124

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A2124 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on TGFB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TGFB1. (Distance between topics and target gene indicate popularity.) TGFB1

* Data provided by citexs.com, for reference only.

Publishing research using A2124? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (6)

Proteins (4)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order