Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TGFB2 Rabbit pAb (A3640)

Publications (12) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - TGFB2 Rabbit pAb (A3640)

Western blot analysis of extracts of various cell lines, using TGFB2 antibody (A3640) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name TGFB2 Rabbit pAb
Catalog No. A3640
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers. A chromosomal translocation that includes this gene is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. Mutations in this gene may be associated with Loeys-Dietz syndrome. This gene encodes multiple isoforms that may undergo similar proteolytic processing.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human TGFB2 (NP_001129071.1).
Sequence SLSTCSTLDMDQFMRKRIEAIRGQILSKLKLTSPPEDYPEPEEVPPEVISIYNSTRDLLQEKASRRAAACERERSDEEYYAKEVYKIDMPPFFPSETVCPVVTTPSGSVGSLCSRQSQVLCGYLDAIPPTFYRPYFRIVRF
Gene ID 7042
Swiss prot P61812
Synonyms LDS4; G-TSF; TGF-beta2; TGFB2
Calculated MW 48kDa
Observed MW 48kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse lung, Mouse heart, Mouse uterus, Rat lung, Rat heart
Cellular location Secreted
Customer validation

WB (Homo sapiens, Gallus gallus, Mus musculus)

IF (Gallus gallus)

IHC (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

TGFB2 Rabbit pAb images

ABclonal:Western blot - TGFB2 Rabbit pAb (A3640)}

Western blot - TGFB2 Rabbit pAb (A3640)

Western blot analysis of extracts of various cell lines, using TGFB2 antibody (A3640) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on TGFB2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TGFB2. (Distance between topics and target gene indicate popularity.) TGFB2

* Data provided by citexs.com, for reference only.

Publishing research using A3640? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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