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TGFB1 Rabbit pAb (A2124)

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ABclonal:Western blot - TGFB1 Rabbit pAb (A2124)
Review
ABclonal: review for TGFB1 Rabbit pAb(A2124)

Overview

Product nameTGFB1 Rabbit pAb
Catalog No.A2124
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-280 of human TGFB1 (NP_000651.3).
SequenceLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRAL
Gene ID7040
Swiss protP01137
SynonymsTGFB1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-β
Calculated MW44kDa
Observed MW44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA-549, Rat heart
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

(Rat heart)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa)

IHC(Mus musculus, Rattus norvegicus, Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - TGFB1 Rabbit pAb (A2124) }

Western blot - TGFB1 Rabbit pAb (A2124)

Western blot analysis of extracts of various cell lines, using TGFB1 antibody (A2124) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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