Products > Catalog Antibodies > Polyclonal Antibodies

TFRC Rabbit pAb (A5865)

Publications (2) Datasheet

ABclonal:Western blot - TFRC Rabbit pAb (A5865)
ABclonal:Immunohistochemistry - TFRC Rabbit pAb (A5865)
ABclonal:Immunohistochemistry - TFRC Rabbit pAb (A5865)
ABclonal:Immunohistochemistry - TFRC Rabbit pAb (A5865)
ABclonal:Immunohistochemistry - TFRC Rabbit pAb (A5865)
ABclonal:Immunofluorescence - TFRC Rabbit pAb (A5865)
ABclonal:Immunoprecipitation - TFRC Rabbit pAb (A5865)

Overview

Product nameTFRC Rabbit pAb
Catalog No.A5865
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human TFRC (NP_001121620.1).
SequenceMMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDF
Gene ID7037
Swiss protP02786
SynonymsTFRC; CD71; IMD46; T9; TFR; TFR1; TR; TRFR; p90
Calculated MW84kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, Raji, HepG2, Jurkat, Mouse spleen, Mouse liver, Rat spleen
Cellular locationCell membrane, Melanosome, Secreted, Single-pass type II membrane protein
Customer validation

WB(Homo sapiens, Rattus norvegicus)

IF(Rattus norvegicus)

ABclonal:Western blot - TFRC Rabbit pAb (A5865) }

Western blot - TFRC Rabbit pAb (A5865)

Western blot analysis of extracts of various cell lines, using TFRC antibody (A5865) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - TFRC Rabbit pAb (A5865) }

Immunohistochemistry - TFRC Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded rat kidney using TFRC antibody (A5865) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TFRC Rabbit pAb (A5865) }

Immunohistochemistry - TFRC Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded human liver cancer using TFRC antibody (A5865) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TFRC Rabbit pAb (A5865) }

Immunohistochemistry - TFRC Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TFRC antibody (A5865) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TFRC Rabbit pAb (A5865) }

Immunohistochemistry - TFRC Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded human placenta using TFRC antibody (A5865) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TFRC Rabbit pAb (A5865) }

Immunofluorescence - TFRC Rabbit pAb (A5865)

Immunofluorescence analysis of L929 cells using TFRC Polyclonal Antibody (A5865) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - TFRC Rabbit pAb (A5865) }

Immunoprecipitation - TFRC Rabbit pAb (A5865)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug TFRC antibody (A5865). Western blot was performed from the immunoprecipitate using TFRC antibody (A5865) at a dilition of 1:1000.

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