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TET2 Rabbit pAb (A5682)

Publications (3) Datasheet

ABclonal:Western blot - TET2 Rabbit pAb (A5682)

Western blot analysis of extracts of various cell lines, using TET2 antibody (A5682) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry of paraffin-embedded rat lung using TET2 antibody (A5682) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry of paraffin-embedded human lung cancer using TET2 antibody (A5682) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TET2 antibody (A5682) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry of paraffin-embedded mouse lung using TET2 antibody (A5682) at dilution of 1:100 (40x lens).

Overview

Product nameTET2 Rabbit pAb
Catalog No.A5682
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1833-2002 of human TET2 (NP_001120680.1).
SequenceVQGVASGAEDNDEVWSDSEQSFLDPDIGGVAVAPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAEKAREKEEECEKYGPDYVPQKSHGKKVKREPAEPHETSEPTYLRFIKSLAERTMSVTTDSTVTTSPYAFTRVTGPYNRYI
Gene ID54790
Swiss protQ6N021
SynonymsMDS; KIAA1546; TET2
Calculated MW130kDa/133kDa/223kDa
Observed MW280KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular location
Customer validation

IF(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

ABclonal:Western blot - TET2 Rabbit pAb (A5682) }

Western blot - TET2 Rabbit pAb (A5682)

Western blot analysis of extracts of various cell lines, using TET2 antibody (A5682) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682) }

Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry of paraffin-embedded rat lung using TET2 antibody (A5682) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682) }

Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry of paraffin-embedded human lung cancer using TET2 antibody (A5682) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682) }

Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TET2 antibody (A5682) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682) }

Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry of paraffin-embedded mouse lung using TET2 antibody (A5682) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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