Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TET2 Rabbit pAb (A5682)

Publications (10) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - TET2 Rabbit pAb (A5682)

Western blot analysis of various lysates using TET2 Rabbit pAb (A5682) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry analysis of TET2 in paraffin-embedded rat lung using TET2 Rabbit pAb (A5682) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry analysis of TET2 in paraffin-embedded human lung cancer using TET2 Rabbit pAb (A5682) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry analysis of TET2 in paraffin-embedded human colon carcinoma using TET2 Rabbit pAb (A5682) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry analysis of TET2 in paraffin-embedded mouse lung using TET2 Rabbit pAb (A5682) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TET2 Rabbit pAb (A5682)

Immunofluorescence analysis of F9 cells using TET2 Rabbit pAb (A5682) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name TET2 Rabbit pAb
Catalog No. A5682
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1833-2002 of human TET2 (NP_001120680.1).
Sequence VQGVASGAEDNDEVWSDSEQSFLDPDIGGVAVAPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAEKAREKEEECEKYGPDYVPQKSHGKKVKREPAEPHETSEPTYLRFIKSLAERTMSVTTDSTVTTSPYAFTRVTGPYNRYI
Gene ID 54790
Swiss prot Q6N021
Synonyms MDS; IMD75; KIAA1546; TET2
Calculated MW 224kDa
Observed MW 280kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Mouse brain, Rat brain
Cellular location nucleus
Customer validation

IF (Homo sapiens, Mus musculus, Sus scrofa)

IHC (Homo sapiens)

WB (Mus musculus, Homo sapiens)

RT-qPCR (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

TET2 Rabbit pAb images

ABclonal:Western blot - TET2 Rabbit pAb (A5682)}

Western blot - TET2 Rabbit pAb (A5682)

Western blot analysis of various lysates using TET2 Rabbit pAb (A5682) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)}

Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry analysis of TET2 in paraffin-embedded rat lung using TET2 Rabbit pAb (A5682) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)}

Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry analysis of TET2 in paraffin-embedded human lung cancer using TET2 Rabbit pAb (A5682) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)}

Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry analysis of TET2 in paraffin-embedded human colon carcinoma using TET2 Rabbit pAb (A5682) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TET2 Rabbit pAb (A5682)}

Immunohistochemistry - TET2 Rabbit pAb (A5682)

Immunohistochemistry analysis of TET2 in paraffin-embedded mouse lung using TET2 Rabbit pAb (A5682) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TET2 Rabbit pAb (A5682)}

Immunofluorescence - TET2 Rabbit pAb (A5682)

Immunofluorescence analysis of F9 cells using TET2 Rabbit pAb (A5682) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TET2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TET2. (Distance between topics and target gene indicate popularity.) TET2

* Data provided by citexs.com, for reference only.

Publishing research using A5682? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Proteins (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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