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TEK Rabbit pAb (A0743)

Publication (1) Datasheet

ABclonal:Western blot - TEK Rabbit pAb (A0743)

Western blot analysis of extracts of HUVEC cells, using TEK antibody (A0743) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TEK Rabbit pAb (A0743)

Immunohistochemistry of paraffin-embedded human lung cancer using TEK antibody (A0743) at dilution of 1:100 (40x lens).

Overview

Product nameTEK Rabbit pAb
Catalog No.A0743
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human TEK (NP_000450.2).
SequenceEIVTLNYLEPRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSHELVTLPESQAPADLGGGKMLLIA
Gene ID7010
Swiss protQ02763
SynonymsTEK; CD202B; GLC3E; TIE-2; TIE2; VMCM; VMCM1; Tie2
Calculated MW109kDa/121kDa/125kDa
Observed MW150KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHUVEC
Cellular locationCell junction, Cell membrane, Cytoplasm, Secreted, Single-pass type I membrane protein, cytoskeleton, focal adhesion
Customer validation

IHC(Homo sapiens)

ABclonal:Western blot - TEK Rabbit pAb (A0743) }

Western blot - TEK Rabbit pAb (A0743)

Western blot analysis of extracts of HUVEC cells, using TEK antibody (A0743) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TEK Rabbit pAb (A0743) }

Immunohistochemistry - TEK Rabbit pAb (A0743)

Immunohistochemistry of paraffin-embedded human lung cancer using TEK antibody (A0743) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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