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TEAD1 Rabbit pAb (A13366)

Datasheet

ABclonal:Western blot - TEAD1 Rabbit pAb (A13366)

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (A13366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - TEAD1 Rabbit pAb (A13366)

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (A13366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - TEAD1 Rabbit pAb (A13366)

Immunohistochemistry of paraffin-embedded rat brain using TEAD1 Rabbit pAb (A13366) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - TEAD1 Rabbit pAb (A13366)

Immunohistochemistry of paraffin-embedded human placenta using TEAD1 Rabbit pAb (A13366) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - TEAD1 Rabbit pAb (A13366)

Immunohistochemistry of paraffin-embedded mouse heart using TEAD1 Rabbit pAb (A13366) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - TEAD1 Rabbit pAb (A13366)

Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (A13366).

ABclonal:Chromatin Immunoprecipitation - TEAD1 Rabbit pAb (A13366)

Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (A13366) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameTEAD1 Rabbit pAb
Catalog No.A13366
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
SequenceAIHNKLGLPGIPRPTFPGAPGFWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPASAPAPSVPAWQGRSIGTTK
Gene ID7003
Swiss protP28347
SynonymsTEAD1; AA; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEF-1
Calculated MW40kDa/47kDa
Observed MW50KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
ChIP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HeLa, Mouse skeletal muscle, Mouse heart, Rat heart
Cellular locationNucleus
ABclonal:Western blot - TEAD1 Rabbit pAb (A13366) }

Western blot - TEAD1 Rabbit pAb (A13366)

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (A13366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - TEAD1 Rabbit pAb (A13366) }

Western blot - TEAD1 Rabbit pAb (A13366)

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (A13366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - TEAD1 Rabbit pAb (A13366) }

Immunohistochemistry - TEAD1 Rabbit pAb (A13366)

Immunohistochemistry of paraffin-embedded rat brain using TEAD1 Rabbit pAb (A13366) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TEAD1 Rabbit pAb (A13366) }

Immunohistochemistry - TEAD1 Rabbit pAb (A13366)

Immunohistochemistry of paraffin-embedded human placenta using TEAD1 Rabbit pAb (A13366) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TEAD1 Rabbit pAb (A13366) }

Immunohistochemistry - TEAD1 Rabbit pAb (A13366)

Immunohistochemistry of paraffin-embedded mouse heart using TEAD1 Rabbit pAb (A13366) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TEAD1 Rabbit pAb (A13366) }

Immunofluorescence - TEAD1 Rabbit pAb (A13366)

Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (A13366).
ABclonal:Chromatin Immunoprecipitation - TEAD1 Rabbit pAb (A13366) }

Chromatin Immunoprecipitation - TEAD1 Rabbit pAb (A13366)

Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (A13366) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

* For research use only. Not for therapeutic or diagnostic purposes.

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