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TCF7L2 Rabbit mAb (A19548)

Datasheet

ABclonal:Western blot - TCF7L2 Rabbit mAb (A19548)
ABclonal:Immunohistochemistry - TCF7L2 Rabbit mAb (A19548)
ABclonal:Immunohistochemistry - TCF7L2 Rabbit mAb (A19548)
ABclonal:Immunohistochemistry - TCF7L2 Rabbit mAb (A19548)

Overview

Product nameTCF7L2 Rabbit mAb
Catalog No.A19548
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]
ImmunogenA synthesized peptide derived from human TCF7L2.
SequenceEmail for sequence
Gene ID6934
Swiss protQ9NQB0
SynonymsTCF-4; TCF4; TCF7L2
Calculated MW68kDa
Observed MW68KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesSW620, HeLa, U-251MG, HepG2
Cellular location
ABclonal:Western blot - TCF7L2 Rabbit mAb (A19548) }

Western blot - TCF7L2 Rabbit mAb (A19548)

Western blot analysis of extracts of various cell lines, using TCF7L2 antibody (A19548) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - TCF7L2 Rabbit mAb (A19548) }

Immunohistochemistry - TCF7L2 Rabbit mAb (A19548)

Immunohistochemistry of paraffin-embedded rat testis using TCF7L2 antibody (A19548) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TCF7L2 Rabbit mAb (A19548) }

Immunohistochemistry - TCF7L2 Rabbit mAb (A19548)

Immunohistochemistry of paraffin-embedded human lung cancer using TCF7L2 antibody (A19548) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TCF7L2 Rabbit mAb (A19548) }

Immunohistochemistry - TCF7L2 Rabbit mAb (A19548)

Immunohistochemistry of paraffin-embedded mouse testis using TCF7L2 antibody (A19548) at dilution of 1:100 (40x lens).

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