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TAZ Rabbit pAb (A15806)

Publication (1) Datasheet

ABclonal:Western blot - TAZ Rabbit pAb (A15806)

Western blot analysis of extracts of various cell lines, using TAZ antibody (A15806) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - TAZ Rabbit pAb (A15806)

Immunofluorescence analysis of U2OS cells using TAZ antibody (A15806) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameTAZ Rabbit pAb
Catalog No.A15806
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TAZ (NP_056287.1).
SequenceLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL
Gene ID25937
Swiss protQ9GZV5
SynonymsWW domain-containing transcription regulator protein 1; Transcriptional coactivator with PDZ-binding motif; TAZ; WWTR1
Calculated MW44kDa
Observed MW55kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesNCI-H460, Mouse lung
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus)

ABclonal:Western blot - TAZ Rabbit pAb (A15806) }

Western blot - TAZ Rabbit pAb (A15806)

Western blot analysis of extracts of various cell lines, using TAZ antibody (A15806) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - TAZ Rabbit pAb (A15806) }

Immunofluorescence - TAZ Rabbit pAb (A15806)

Immunofluorescence analysis of U2OS cells using TAZ antibody (A15806) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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