Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Publications (2) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Symmetric DiMethyl-Histone H4-R3 Rabbit pAb
ABclonal: - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H4-R3 antibody (A3159).

ABclonal:Immunohistochemistry - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Immunohistochemistry of paraffin-embedded mouse brain using Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H4-R3 antibody (A3159). Blue: DAPI for nuclear staining.

Overview

Product nameSymmetric DiMethyl-Histone H4-R3 Rabbit pAb
Catalog No.A3159
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

ImmunogenA synthetic symmetric dimethylated peptide around R3 of human Histone H4 (NP_003529.1).
SequenceSGRGK
Gene ID8370
Swiss protP62805
SynonymsFO108; H4; H4/n; H4F2; H4FN; HIST2H4; Histone H4; HIST1H4A; HIST2H4A
Calculated MW11kDa
Observed MWRefer to figures

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • DB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyImmunohistochemistry    
Positive samples
Cellular locationChromosome, Nucleus
Customer validation

 WB(Danio rerio )

WB(Homo sapiens)

Research Area

Symmetric DiMethyl-Histone H4-R3 Rabbit pAb images

ABclonal: - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)}

- Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H4-R3 antibody (A3159).
ABclonal:Immunohistochemistry - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)}

Immunohistochemistry - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Immunohistochemistry of paraffin-embedded mouse brain using Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)}

Immunofluorescence - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H4-R3 antibody (A3159). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on Histone H4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Histone H4. (Distance between topics and target gene indicate popularity.) Histone H4

* Data provided by citexs.com, for reference only.

Publishing research using A3159? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (20)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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