Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Tested applications:WB IHC IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal: - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H4-R3 antibody (A3159).

ABclonal:Immunohistochemistry - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Immunohistochemistry of paraffin-embedded mouse brain using Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H4-R3 antibody (A3159). Blue: DAPI for nuclear staining.

Overview

Product name	Symmetric DiMethyl-Histone H4-R3 Rabbit pAb
Catalog No.	A3159
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

Immunogen	A synthetic symmetric dimethylated peptide around R3 of human Histone H4 (NP_003529.1).
Sequence	SGRGK
Gene ID	8370
Swiss prot	P62805
Synonyms	FO108; H4; H4/n; H4F2; H4FN; HIST2H4; Histone H4; HIST1H4A; HIST2H4A
Calculated MW	11kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range)
Tested applications	WB IHC IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	DB 1:500 - 1:2000 IHC 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application key	Immunohistochemistry
Positive samples
Cellular location	Chromosome, Nucleus
Customer validation	WB(Danio rerio ) WB(Homo sapiens)

Research Area

Symmetric DiMethyl-Histone H4-R3 Rabbit pAb images

- Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H4-R3 antibody (A3159).

Immunohistochemistry - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Immunofluorescence - Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159)

Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H4-R3 antibody (A3159). Blue: DAPI for nuclear staining.

Product Protocols

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Related Targets

Based on existing publications, the following genes are closely related to research on Histone H4. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to Histone H4. (Distance between topics and target gene indicate popularity.) Histone H4

* Data provided by citexs.com, for reference only.