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Survivin Polyclonal Antibody (A1551)

Review (1)Publications (2) Datasheet

ABclonal:Western blot - Survivin Polyclonal Antibody (A1551)
ABclonal:Immunofluorescence - Survivin Polyclonal Antibody (A1551)
Review
ABclonal: review for Survivin Polyclonal Antibody(A1551)

Overview

Product nameSurvivin Polyclonal Antibody
Catalog No.A1551
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human Survivin (NP_001159.2).
SequenceMGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAEKVRRAIEQLAAMD
Gene ID332
Swiss protO15392
SynonymsBIRC5; API4; EPR-1; baculoviral IAP repeat containing 5
Calculated MW8kDa/13kDa/15kDa/16kDa/18kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesDU145, K-562, ES-2
Cellular locationChromosome, Cytoplasm, Midbody, Nucleus, centromere, cytoskeleton, kinetochore, spindle
Customer validation

WB(Rat, Mouse)

ABclonal:Western blot - Survivin Polyclonal Antibody (A1551) }

Western blot - Survivin Polyclonal Antibody (A1551)

Western blot analysis of extracts of various cell lines, using Survivin antibody (A1551) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunofluorescence - Survivin Polyclonal Antibody (A1551) }

Immunofluorescence - Survivin Polyclonal Antibody (A1551)

Immunofluorescence analysis of U2OS cells using Survivin antibody (A1551). Blue: DAPI for nuclear staining.

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