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Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Publications (3) Datasheet

ABclonal:Western blot - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Western blot analysis of extracts of various cell lines, using Sonic Hedgehog (Sonic Hedgehog (Shh)) antibody (A7726) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.

ABclonal:Immunohistochemistry - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Immunohistochemistry of paraffin-embedded rat kidney using Sonic Hedgehog (Sonic Hedgehog (Shh)) antibody (A7726) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Immunohistochemistry of paraffin-embedded rat lung using Sonic Hedgehog (Sonic Hedgehog (Shh)) antibody (A7726) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Immunofluorescence analysis of HeLa cells using Sonic Hedgehog (Sonic Hedgehog (Shh)) Polyclonal Antibody (A7726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Immunofluorescence analysis of NIH-3T3 cells using Sonic Hedgehog (Sonic Hedgehog (Shh)) Polyclonal Antibody (A7726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameSonic Hedgehog (Shh) Rabbit pAb
Catalog No.A7726
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
ImmunogenA synthetic peptide of human Sonic Hedgehog (Sonic Hedgehog (Shh))
SequenceEmail for sequence
Gene ID6469
Swiss protQ15465
SynonymsSHH; HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
Calculated MW49kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, A-549
Cellular locationCell membrane, Lipid-anchor, Secreted, extracellular space
Customer validation

WB(Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - Sonic Hedgehog (Shh) Rabbit pAb (A7726) }

Western blot - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Western blot analysis of extracts of various cell lines, using Sonic Hedgehog (Sonic Hedgehog (Shh)) antibody (A7726) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
ABclonal:Immunohistochemistry - Sonic Hedgehog (Shh) Rabbit pAb (A7726) }

Immunohistochemistry - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Immunohistochemistry of paraffin-embedded rat kidney using Sonic Hedgehog (Sonic Hedgehog (Shh)) antibody (A7726) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Sonic Hedgehog (Shh) Rabbit pAb (A7726) }

Immunohistochemistry - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Immunohistochemistry of paraffin-embedded rat lung using Sonic Hedgehog (Sonic Hedgehog (Shh)) antibody (A7726) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Sonic Hedgehog (Shh) Rabbit pAb (A7726) }

Immunofluorescence - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Immunofluorescence analysis of HeLa cells using Sonic Hedgehog (Sonic Hedgehog (Shh)) Polyclonal Antibody (A7726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Sonic Hedgehog (Shh) Rabbit pAb (A7726) }

Immunofluorescence - Sonic Hedgehog (Shh) Rabbit pAb (A7726)

Immunofluorescence analysis of NIH-3T3 cells using Sonic Hedgehog (Sonic Hedgehog (Shh)) Polyclonal Antibody (A7726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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