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α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Publication (1) Datasheet

ABclonal:Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Western blot analysis of extracts of various cell lines, using α-Smooth Muscle Actin (ACTA2) antibody (A17910) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Immunohistochemistry of paraffin-embedded mouse liver using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Immunofluorescence analysis of smooth muscle of human colon carcinoma using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Immunofluorescence analysis of mouse colon using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Immunofluorescence analysis of rat colon using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameα-Smooth Muscle Actin (ACTA2) Rabbit mAb
Catalog No.A17910
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
ImmunogenA synthesized peptide derived from human α-Smooth Muscle Actin (ACTA2)
SequenceEmail for sequence
Gene ID59
Swiss protP62736
SynonymsAAT6; ACTSA; MYMY5; ACTA2; actin
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRD, Mouse large intestine, Mouse heart, Mouse brain, Rat lung, Rat heart
Cellular locationCytoplasm, cytoskeleton
Customer validation

IHC(Mus musculus)

ABclonal:Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) }

Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Western blot analysis of extracts of various cell lines, using α-Smooth Muscle Actin (ACTA2) antibody (A17910) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) }

Immunohistochemistry - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Immunohistochemistry of paraffin-embedded mouse liver using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) }

Immunofluorescence - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Immunofluorescence analysis of smooth muscle of human colon carcinoma using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) }

Immunofluorescence - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Immunofluorescence analysis of mouse colon using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) }

Immunofluorescence - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Immunofluorescence analysis of rat colon using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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