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Serotonin transporter Rabbit pAb (A14171)

Publication (1) Datasheet

ABclonal:Western blot - Serotonin transporter Rabbit pAb (A14171)

Western blot analysis of extracts of various cell lines, using Serotonin transporter Polyclonal Antibody (A14171) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - Serotonin transporter Rabbit pAb (A14171)

Immunohistochemistry of paraffin-embedded rat testis using Serotonin transporter Rabbit pAb (A14171) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Serotonin transporter Rabbit pAb (A14171)

Immunohistochemistry of paraffin-embedded mouse testis using Serotonin transporter Rabbit pAb (A14171) at dilution of 1:100 (40x lens).

Overview

Product nameSerotonin transporter Rabbit pAb
Catalog No.A14171
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human Serotonin transporter (NP_001036.1).
SequenceAVLTVALIEAVAVSWFYGITQFCRDVKEMLGFSPGWFWRICWVAISPLFLLFIICSFLMSPPQLRLFQYNYPYWSIILGYCIGTSSFICIPTYIAYRLIITPGTFKERIIKSITPETPTEIPCGDIRLNAV
Gene ID6532
Swiss protP31645
Synonyms5-HTT; 5-HTTLPR; 5HTT; HTT; OCD1; SERT; SERT1; hSERT; SLC6A4
Calculated MW70kDa/74kDa
Observed MW75KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, THP-1, HUVEC, Mouse brain, Rat brain
Cellular locationCell membrane, Endomembrane system, Endosome membrane, Multi-pass membrane protein
Customer validation

WB(Zea mays)

IF(Zea mays)

ABclonal:Western blot - Serotonin transporter Rabbit pAb (A14171) }

Western blot - Serotonin transporter Rabbit pAb (A14171)

Western blot analysis of extracts of various cell lines, using Serotonin transporter Polyclonal Antibody (A14171) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - Serotonin transporter Rabbit pAb (A14171) }

Immunohistochemistry - Serotonin transporter Rabbit pAb (A14171)

Immunohistochemistry of paraffin-embedded rat testis using Serotonin transporter Rabbit pAb (A14171) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Serotonin transporter Rabbit pAb (A14171) }

Immunohistochemistry - Serotonin transporter Rabbit pAb (A14171)

Immunohistochemistry of paraffin-embedded mouse testis using Serotonin transporter Rabbit pAb (A14171) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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